Distribution and accumulation of dietary ergothioneine and its metabolites in mouse tissues
| Autor: | Barry Halliwell, Irwin K. Cheah, Terry Shze Keong Yew, Richard Ming Yi Tang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Antioxidant medicine.medical_treatment lcsh:Medicine Administration Oral medicine.disease_cause Antioxidants Article 03 medical and health sciences chemistry.chemical_compound Tandem Mass Spectrometry Oral administration In vivo medicine Animals lcsh:Science Whole blood Kidney Multidisciplinary 030102 biochemistry & molecular biology Chemistry lcsh:R Animal Structures Ergothioneine In vitro Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Biochemistry lcsh:Q Oxidative stress Chromatography Liquid |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-15 (2018) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-20021-z |
| Popis: | L-ergothioneine (ET) is a diet-derived amino acid that accumulates at high concentrations in animals and humans. Numerous studies have highlighted its antioxidant abilities in vitro, and possible cytoprotective capabilities in vivo. We investigated the uptake and distribution of ET in various organs by a highly sensitive and specific liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) technique, both before and after oral administration of pure ET (35 and 70 mg/kg/day for 1, 7, and 28 days) to male C57BL6J mice. ET primarily concentrates in the liver and whole blood, and also in spleen, kidney, lung, heart, intestines, eye, and brain tissues. Strong correlations were found between ET and its putative metabolites - hercynine, ET-sulfonate (ET-SO3H), and S-methyl ET. Hercynine accumulates in the brain after prolonged ET administration. This study demonstrates the uptake and distribution of ET and provides a foundation for future studies with ET to target oxidative damage in a range of tissues in human diseases. |
| Databáze: | OpenAIRE |
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