Capillary electrophoresis coupled to electrospray mass spectrometry through a coaxial sheath flow interface and semi-permanent phospholipid coating for the determination of oligosaccharides labeled with 1-aminopyrene-3,6,8-trisulfonic acid
| Autor: | Lisa A. Holland, Ted J. Langan |
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| Rok vydání: | 2012 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Electrospray mass spectrometry Capillary action Clinical Biochemistry Phospholipid Oligosaccharides engineering.material Mass spectrometry Biochemistry Analytical Chemistry chemistry.chemical_compound Capillary electrophoresis Coating Enzymatic hydrolysis Phospholipids Pyrenes Chromatography Chemistry Electrophoresis Capillary Hydrogen-Ion Concentration beta-Galactosidase engineering lipids (amino acids peptides and proteins) Electroosmosis Glucan 1 4-alpha-Glucosidase Coaxial |
| Zdroj: | ELECTROPHORESIS. 33:607-613 |
| ISSN: | 0173-0835 |
| DOI: | 10.1002/elps.201100449 |
| Popis: | For the first time, a semi-permanent phospholipid coating is utilized in capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS). Although phospholipids in free solution are generally avoided in ESI, they did not interfere with the detection of linear and branched oligosaccharides using ESI operated in negative mode. The CE and ESI were coupled using a coaxial sheath flow interface. The separation was operated in reversed polarity and the electroosmotic flow was effectively suppressed by the phospholipid coating. The method was characterized with linear oligosaccharides and used to monitor the enzymatic hydrolysis of maltooligosaccharides with α-amyloglucosidase. Branched oligosaccharides were separated and detected with the system. The enzyme β1-4 galactosidase was used to distinguish branched isomeric oligosaccharides derived from asialofetuin. |
| Databáze: | OpenAIRE |
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