YY1 Is Required for Posttranscriptional Stability of SOX2 and OCT4 Proteins
| Autor: | Kun Zhang, Jacob Hiller, Mary C. Wallingford, Jesse Mager |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gene isoform Biology Mice 03 medical and health sciences SOX2 medicine Animals Inner cell mass Blastocyst Transcription factor Research Articles YY1 Transcription Factor Mice Knockout Neuroectoderm Protein Stability YY1 SOXB1 Transcription Factors fungi Cell Biology Embryonic stem cell Cell biology 030104 developmental biology medicine.anatomical_structure embryonic structures Cancer research Female biological phenomena cell phenomena and immunity Octamer Transcription Factor-3 Developmental Biology Biotechnology |
| Zdroj: | Cellular Reprogramming. 19:263-269 |
| ISSN: | 2152-4998 2152-4971 |
| DOI: | 10.1089/cell.2017.0002 |
| Popis: | Yinyang1 (YY1) participates in protein-DNA, protein-RNA, and protein-protein interactions and regulates developmental processes and disease mechanisms. YY1 interactions regulate a range of important biological functions, including oocyte maturation, epithelial to mesenchymal transition, and vascular endothelial growth factor (VEGF) signaling. We tested the hypothesis that YY1 is required for inner cell mass (ICM) lineage commitment during preimplantation development. In this study, we document gene expression patterns and protein localization of key transcription factors in Yy1 global, tissue-specific, and dsRNA-mediated knockout/down embryos. YY1 protein was found in cells of preimplantation and peri-implantation embryos, and adult tissues where two isoforms are observed. In the absence of YY1, OCT4 and SOX2 protein were lost in the ICM during preimplantation and naive neuroectoderm during gastrulation stages, yet no difference in Oct4 or Sox2 mRNA levels was observed. The loss of OCT4 and SOX2 protein occurred specifically in cells that normally express both OCT4 and SOX2 protein. These observations support a role for YY1 meditating and/or regulating the interaction of OCT4 and SOX2 at a posttranscriptional level. Our results suggest that distinct mechanisms of YY1-mediated molecular regulation are present in the early embryo, and may offer insight to promote lineage commitment in in vitro cell lines. |
| Databáze: | OpenAIRE |
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