Cytochrome P450‐catalyzed biosynthesis of furanoditerpenoids in the bioenergy crop switchgrass ( Panicum virgatum L.)
| Autor: | Yuxuan Chen, Kyle A. Pelot, Kira Tiedge, John T. Lovell, Andrew Muchlinski, Danielle Davisson, Lisl Chew, Jason S. Fell, Philipp Zerbe, Meirong Jia, Justin B. Siegel |
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| Rok vydání: | 2021 |
| Předmět: |
cytochrome P450 monooxygenase
plant specialized metabolism Metabolite Plant Biology & Botany Plant Biology Plant Science Panicum Plant Roots chemistry.chemical_compound Cytochrome P-450 Enzyme System Biosynthesis Catalytic Domain Genetics Plant Proteins Biological Products Genome biology diterpenoid biosynthesis fungi Mutagenesis food and beverages Active site Cytochrome P450 Plant Cell Biology Monooxygenase biology.organism_classification Biosynthetic Pathways plant natural products Panicum virgatum chemistry Biochemistry Biocatalysis biology.protein Biochemistry and Cell Biology Diterpenes Diterpene Genome Plant |
| Zdroj: | The Plant journal : for cell and molecular biology, vol 108, iss 4 |
| ISSN: | 1365-313X 0960-7412 |
| Popis: | Specialized diterpenoid metabolites are important mediators of plant-environment interactions in monocot crops. To understand metabolite functions in plant environmental adaptation that ultimately can enable crop improvement strategies, a deeper knowledge of the underlying species-specific biosynthetic pathways is required. Here, we report the genomics-enabled discovery of five cytochrome P450 monooxygenases (CYP71Z25-CYP71Z29) that form previously unknown furanoditerpenoids in the monocot bioenergy crop Panicum virgatum (switchgrass). Combinatorial pathway reconstruction showed that CYP71Z25-CYP71Z29 catalyze furan ring addition directly to primary diterpene alcohol intermediates derived from distinct class II diterpene synthase products. Transcriptional co-expression patterns and the presence of select diterpenoids in switchgrass roots support the occurrence of P450-derived furanoditerpenoids in planta. Integrating molecular dynamics, structural analysis and targeted mutagenesis identified active site determinants that contribute to the distinct catalytic specificities underlying the broad substrate promiscuity of CYP71Z25-CYP71Z29 for native and non-native diterpenoids. |
| Databáze: | OpenAIRE |
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