Detection of neuritic plaques in Alzheimer's disease by magnetic resonance microscopy
| Autor: | Helene Benveniste, G. A. Johnson, Kim Kr, Christine M. Hulette, Gillian Einstein |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Pathology
medicine.medical_specialty Plaque Amyloid computer.software_genre Silver stain Voxel Alzheimer Disease medicine Humans Senile plaques Coloring Agents Aged Aged 80 and over Multidisciplinary medicine.diagnostic_test Magnetic resonance microscopy Chemistry Magnetic resonance imaging Hippocampal sulcus Anatomy Middle Aged Biological Sciences Granule cell medicine.disease Magnetic Resonance Imaging medicine.anatomical_structure Alzheimer's disease Protons computer |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 96(24) |
| ISSN: | 0027-8424 |
| Popis: | Magnetic resonance microscopy (MRM) theoretically provides the spatial resolution and signal-to-noise ratio needed to resolve neuritic plaques, the neuropathological hallmark of Alzheimer’s disease (AD). Two previously unexplored MR contrast parameters, T2* and diffusion, are tested for plaque-specific contrast to noise. Autopsy specimens from nondemented controls ( n = 3) and patients with AD ( n = 5) were used. Three-dimensional T2* and diffusion MR images with voxel sizes ranging from 3 × 10 −3 mm 3 to 5.9 × 10 −5 mm 3 were acquired. After imaging, specimens were cut and stained with a microwave king silver stain to demonstrate neuritic plaques. From controls, the alveus, fimbria, pyramidal cell layer, hippocampal sulcus, and granule cell layer were detected by either T2* or diffusion contrast. These structures were used as landmarks when correlating MRMs with histological sections. At a voxel resolution of 5.9 × 10 −5 mm 3 , neuritic plaques could be detected by T2*. The neuritic plaques emerged as black, spherical elements on T2* MRMs and could be distinguished from vessels only in cross-section when presented in three dimension. Here we provide MR images of neuritic plaques in vitro . The MRM results reported provide a new direction for applying this technology in vivo . Clearly, the ability to detect and follow the early progression of amyloid-positive brain lesions will greatly aid and simplify the many possibilities to intervene pharmacologically in AD. |
| Databáze: | OpenAIRE |
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