The Shaping of a Polyvalent and Highly Individual T-Cell Repertoire in the Bone Marrow of Breast Cancer Patients
| Autor: | Florian Schütz, C Sohn, Joanna Förster, Volker Schirrmacher, Philipp Beckhove, Nora Sommerfeldt |
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| Rok vydání: | 2006 |
| Předmět: |
Cancer Research
T-Lymphocytes Bone Marrow Cells Breast Neoplasms Enzyme-Linked Immunosorbent Assay complex mixtures Breast cancer Immune system Antigen Antigens Neoplasm Reference Values HLA-A2 Antigen parasitic diseases Humans Medicine Amino Acid Sequence T cell repertoire HLA-A Antigens business.industry ELISPOT Repertoire Cancer medicine.disease Peptide Fragments digestive system diseases medicine.anatomical_structure Oncology Immunology Female Bone marrow business |
| Zdroj: | Cancer Research. 66:8258-8265 |
| ISSN: | 1538-7445 0008-5472 |
| Popis: | We analyzed the T-cell repertoires from the bone marrow of 39 primary operated breast cancer patients and 11 healthy female donors for the presence and frequencies of spontaneously induced effector/memory T lymphocytes with peptide-HLA-A2-restricted reactivity against 10 breast tumor-associated antigens (TAA) and 3 normal breast tissue–associated antigens by short-term IFN-γ enzyme-linked immunospot (ELISpot) analysis. Sixty-seven percent of the patients recognized TAAs with a mean frequency of 144 TAA reactive cells per 106 T cells. These patients recognized simultaneously an average of 47% of the tested TAAs. The T-cell repertoire was highly polyvalent and exhibited pronounced interindividual differences in the pattern of TAAs recognized by each patient. Strong differences of reactivity were noticed between TAAs, ranging from 100% recognition of prostate-specific antigenp141-149 to only 25% recognition of MUC1p12-20 or Her-2/neup369-377. In comparison with TAAs, reactivity to normal breast tissue–associated antigens was lower with respect to the proportions of responding patients (30%) and recognized antigens (27%), with a mean frequency of only 85/106 T cells. Healthy individuals also contained TAA-reactive T cells but this repertoire was more restricted and the frequencies were in the same range as T cells reacting to normal breast tissue–associated antigens. Our data show a highly individual T-cell repertoire for recognition of TAAs in breast cancer patients. This has potential relevance for T-cell immune diagnostics, for tumor vaccine design, and for predicting immune responsiveness. (Cancer Res 2006; 66(16): 8258-65) |
| Databáze: | OpenAIRE |
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