Cloning of some heat shock proteins genes for further transcriptional study of Planktothrix agardhii exposed to abiotic stress
| Autor: | Chi Thi Du Tran, Katia Comte, Cécile Bernard |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Genetics
DNA Bacterial biology Microcystins Abiotic stress Operon Molecular Sequence Data General Medicine GroES Sequence Analysis DNA biology.organism_classification Cyanobacteria Microbiology Planktothrix GroEL Stress Physiological Heat shock protein Cellular stress response HSP60 Cloning Molecular Heat-Shock Proteins |
| Zdroj: | Folia microbiologica. 60(4) |
| ISSN: | 1874-9356 |
| Popis: | Planktothrix agardhii is one of the freshwater cyanobacteria that can produce the hepatotoxin microcystins (MC)—a real threat to human and animal health. Knowledge of the biological role of MC in producing organisms is highly desired to understand the driving force of MC production. Recently, emerging evidences have suggested that MC may have protective role in cells facing environmental stress. If this is true, one should expect differences in the cellular protective mechanisms between MC-containing and MC-deficient mutant strains. To test this hypothesis, it would be essential to investigate the consequences of the loss of MC in Planktothrix in the transcriptional responses of its heat shock proteins (Hsps) to abiotic stresses—an important component of cellular stress response. However, a crucial first step is prerequisite for the isolation of hsp genes here, as the genome of Planktothrix has not been fully published. Therefore, we have successfully isolated four hsp genes including clpC (hsp100), htpG (hsp90), groEL (hsp60), and groES (hsp10) from Planktothrix agardhii PCC 7805 using ramped annealing PCR (RAN-PCR) with consensus-degenerate hybrid oligonucleotide primers (CODEHOP) and annealing control primer (ACP) system. In addition, some putative regulatory sequences found in the upstream region of groESL operon of Planktothrix agardhii were also discussed. |
| Databáze: | OpenAIRE |
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