Oxidative stress and DNA damage responses in rat and mouse lung to inhaled carbon nanoparticles
| Autor: | Roel P. F. Schins, Agnes M. Scherbart, Miriam E. Gerlofs-Nijland, Anton Wessels, Agnes W. Boots, Frederik-Jan van Schooten, Flemming R. Cassee, Catrin Albrecht, Damien van Berlo, Kirsten Gerloff |
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| Přispěvatelé: | RS: NUTRIM - R3 - Chronic inflammatory disease and wasting, RS: NUTRIM - R4 - Gene-environment interaction, Farmacologie en Toxicologie |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
Pathology medicine.medical_specialty DNA damage Biomedical Engineering Respiratory Mucosa Biology Toxicology medicine.disease_cause XRCC1 Mice medicine Animals RNA Messenger Lung Inhalation exposure Air Pollutants Inhalation Exposure Inhalation medicine.diagnostic_test Dose-Response Relationship Drug Molecular biology Carbon Rats Inbred F344 Rats Mice Inbred C57BL Oxidative Stress Bronchoalveolar lavage medicine.anatomical_structure Toxicity Nanoparticles Female Bronchoalveolar Lavage Fluid Oxidative stress DNA Damage |
| Zdroj: | Nanotoxicology, 5(1), 66-78. Informa Healthcare |
| ISSN: | 1743-5390 |
| DOI: | 10.3109/17435390.2010.494773 |
| Popis: | We have investigated whether short-term nose-only inhalation exposure to electric spark discharge-generated carbon nanoparticles ( approximately 60 nm) causes oxidative stress and DNA damage responses in the lungs of rats (152 mug/m(3); 4 h) and mice (142 mug/m(3); 4 h, or three times 4 h). In both species, no pulmonary inflammation and toxicity were detected by bronchoalveolar lavage or mRNA expression analyses. Oxidative DNA damage (measured by fpg-comet assay), was also not increased in mouse whole lung tissue or isolated lung epithelial cells from rat. In addition, the mRNA expressions of the DNA base excision repair genes OGG1, DNA Polbeta and XRCC1 were not altered. However, in the lung epithelial cells isolated from the nanoparticle-exposed rats a small but significant increase in APE-1 mRNA expression was measured. Thus, short-term inhalation of carbon nanoparticles under the applied exposure regimen, does not cause oxidative stress and DNA damage in the lungs of healthy mice and rats. |
| Databáze: | OpenAIRE |
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