Plasma Thiols Levels in Alzheimer's Disease Mice under Diet-Induced Hyperhomocysteinemia: Effect of S-Adenosylmethionine and Superoxide-Dismutase Supplementation
| Autor: | Jacopo Gervasoni, Sigfrido Scarpa, Claudia Desiderio, Bruno Giardina, Silvia Persichilli, Rosaria A. Cavallaro, Vincenzina Nicolia, Alessandra Di Napoli, Andrea Fuso |
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| Rok vydání: | 2015 |
| Předmět: |
S-Adenosylmethionine
Antioxidant Homocysteine medicine.medical_treatment medicine.disease_cause Amyloid beta-Protein Precursor Mice chemistry.chemical_compound Chromatography High Pressure Liquid Chromatography biology Chemistry superoxide-dismutase General Neuroscience General Medicine Alzheimer's disease Glutathione Psychiatry and Mental health Clinical Psychology Biochemistry Hyperhomocysteinemia medicine.medical_specialty Mice Transgenic Superoxide dismutase Thiols Alzheimer Disease Internal medicine medicine Animals Humans Sulfhydryl Compounds Settore BIO/10 - BIOCHIMICA Superoxide Dismutase homocysteine medicine.disease Mice Inbred C57BL Disease Models Animal B vitamins Endocrinology Mutation Alzheimer biology.protein HPLC Geriatrics and Gerontology Oxidative stress Cysteine |
| Zdroj: | Journal of Alzheimer's disease 44 (2015): 1323–1331. doi:10.3233/JAD-142391 info:cnr-pdr/source/autori:Persichilli, Silvia; Gervasoni, Jacopo; Di Napoli, Alessandra; Fuso, Andrea; Nicolia, Vincenzina; Giardina, Bruno; Scarpa, Sigfrido; Desiderio, Claudia; Cavallaro, Rosaria A./titolo:Plasma Thiols Levels in Alzheimer's Disease Mice under Diet-Induced Hyperhomocysteinemia: Effect of S-Adenosylmethionine and Superoxide-Dismutase Supplementation/doi:10.3233%2FJAD-142391/rivista:Journal of Alzheimer's disease/anno:2015/pagina_da:1323/pagina_a:1331/intervallo_pagine:1323–1331/volume:44 |
| ISSN: | 1875-8908 1387-2877 |
| DOI: | 10.3233/jad-142391 |
| Popis: | Widely confirmed reports were published on association between hyperhomocysteinemia, B vitamin deficiency, oxidative stress, and amyloid-beta in Alzheimer's disease (AD). Homocysteine, cysteine, cysteinylglycine and glutathione are metabolically interrelated thiols that may be potential indicators of health status and disease risk; they all participate in the metabolic pathway of homocysteine. Previous data obtained in one of our laboratories showed that B vitamin deficiency induced exacerbation of AD-like features in TgCRND8 AD mice; these effects were counteracted by S-adenosylmethionine (SAM) supplementation, through the modulation of DNA methylation and antioxidant pathways. Since the cellular response to oxidative stress typically involves alteration in thiols content, a rapid and sensitive HPLC method with fluorescence detection was here used to evaluate the effect of SAM and superoxide-dismutase (SOD) supplementation on thiols level in plasma, in TgCRND8 mice. The quantitative data obtained from HPLC analysis of mice plasma samples showed significant decrease of thiols level when the B vitamin deficient diet was supplemented with SAM + SOD and SOD alone, the latter showing the greatest effect. All these considerations point out the measurement of plasma thiols concentration as a powerful tool of relevance for all clinical purposes involving the evaluation of oxidative stress. The coupling of HPLC with fluorimetric detection, here used, provided a strong method sensitivity allowing thiols determination at very low levels. |
| Databáze: | OpenAIRE |
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