In Vivo and In Vitro Bioassays for Porcine Ovarian Inhibin and Its Partial Purification
| Autor: | Takashi Minegishi, Kaoru Miyamoto, Yoshihisa Hasegawa, Katsumi Yazaki, Chiaki Yazaki, Masao Igarashi |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
endocrine system Swine Isoelectric focusing Ovary Obstetrics and Gynecology Luteinizing Hormone Biology Follicular fluid Molecular biology Rats Sepharose Follicle-stimulating hormone medicine.anatomical_structure Anterior pituitary In vivo medicine Animals Bioassay Biological Assay Female Inhibins Follicle Stimulating Hormone Luteinizing hormone Cells Cultured |
| Zdroj: | Asia-Oceania Journal of Obstetrics and Gynaecology. 8:317-325 |
| ISSN: | 0389-2328 |
| DOI: | 10.1111/j.1447-0756.1982.tb00582.x |
| Popis: | Inhibin activity in porcine follicular fluid (pFF) was quantitatively assayed in both in vivo and in vitro bioassay systems. Post-castration rise of serum FSH in immature male rats was suppressed dose-dependently by a single ip injection (0.125 to 1 ml) of steroid-free pFF. Rat anterior pituitary cell culture was used as an in vitro assay system, in which both release and synthesis of FSH were suppressed dose-dependently in 0.0125 to 0.4 μl/ml of steroid-free pFF. The minimum detectable dose of pFF in vitro was 0.025 μl/ml and the dose required to produce a half-maximal suppression (ED50) was 0.06 μl/ml. Inhibin was partially purified by ammonium sulphate precipitation, DEAE Sephadex ion-exchange chromatography and Sepharose 6B gel filtration. However, relative specific activity was not increased due to a large loss of inhibin activity. In this respect, isoelectric focusing was very useful for the purification of inhibin. |
| Databáze: | OpenAIRE |
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