Myeloperoxidase gene expression in infant leukemia: a Pediatric Oncology Group Study
Autor: | Garth E. Austin, Jeanette Pullen, Sherif R. Zaki, Andrew J. Carroll, Jonathan J. Shuster, Muxiang Zhou, Michael J. Borowitz, Carlos S. Alvarado, Eric D. Austin |
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Rok vydání: | 1998 |
Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Myeloid Pilot Projects Gene Expression Regulation Enzymologic Immunophenotyping Acute lymphocytic leukemia Gene expression Antineoplastic Combined Chemotherapy Protocols medicine Humans Northern blot DNA Primers Peroxidase Regulation of gene expression biology Infant Newborn Antibodies Monoclonal Infant Hematology medicine.disease Molecular biology Burkitt Lymphoma Gene Expression Regulation Neoplastic medicine.anatomical_structure Treatment Outcome Oncology Myeloperoxidase Karyotyping biology.protein Immunohistochemistry Female |
Zdroj: | Leukemialymphoma. 29(1-2) |
ISSN: | 1042-8194 |
Popis: | A high incidence of co-expression of myeloid-associated antigens in infant B-precursor Acute Lymphocytic Leukemia (B-ALL) has been reported, but the significance of this finding is uncertain. To further assess myeloid differentiation and its prognostic significance in this disease, we investigated the frequency of myeloperoxidase (MPO) gene expression in the blast cells from 43 infants with B-ALL registered in a Pediatric Oncology Group (POG) Pilot Study for Treatment of Infant ALL, utilizing a molecular probe for detection of MPO messenger RNA (mRNA) by Northern blot hybridization and a monoclonal antibody to detect MPO-protein by immunohistochemical staining. Sufficient RNA for Northern blot was extracted from 32 bone marrow or blood samples. In two cases, MPO mRNA was determined by a reverse transcriptase-polymerase chain reaction assay and was negative in both cases. MPO-specific transcripts (MPO+) were present in 19 of 34 (56%) samples analyzed. Immunoreactive MPO protein was positive in 13 of the 20 (65%) patients studied. No correlation was found between MPO gene expression and clinical or laboratory features, karyotypic patterns or clinical outcome. The high frequency of MPO gene expression demonstrated in this study suggests that leukemogenic events in many cases of infant B-ALL appear to involve a pluripotent stem cell not yet fully committed to lymphoid differentiation. |
Databáze: | OpenAIRE |
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