Modulation of complex coordinated molecular signaling by 5HT and a cocktail of inhibitors leads to ovarian maturation of Penaeus monodon in captivity
| Autor: | Bharathi Raja Subramaniya, Soumya Iyer, Thiyagu Mani, Niranjali Devaraj Sivasithamparam, Halagowder Devaraj |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Serotonin Eyestalk ablation Penaeus monodon 03 medical and health sciences Vitellogenin Vitellogenins 0302 clinical medicine Penaeidae Genetics Animals Enzyme Inhibitors Phosphorylation Protein kinase A Protein kinase B 030219 obstetrics & reproductive medicine biology Kinase Ovary Gene Expression Regulation Developmental Cell Biology biology.organism_classification Cell biology 030104 developmental biology biology.protein Female Developmental Biology MPF complex Signal Transduction |
| Zdroj: | Molecular reproduction and development. 86(5) |
| ISSN: | 1098-2795 |
| Popis: | In aquaculture practices, prawn cultivation holds the major share and Penaeus monodon is the main species cultured. The decline in production of P. monodon is mainly due to the limited availability of domesticated broodstock, which is attributed to its reproductive cycle, controlled by complex coordinated signaling mechanisms. Unilateral eyestalk ablation of domesticated females held in captivity is done to induce ovary development, which has certain disadvantages, including a high rate of mortality. Thus, developing alternative techniques for eyestalk ablation in captive broodstock is necessary to induce maturation of ovary. This study exemplifies the role of 5HT along with a cocktail of inhibitors (U0126, Rp-cAMP, and LY294002) in inducing ovarian maturation. In this study, inhibition of pERK by U0126 inhibited vitellogenesis-inhibiting hormone (VIH), which in turn led to the overexpression of vitellogenin. 5HT induces steroidogenesis (estradiol-17β) through induction of the gonadotropin-releasing hormone by activating calcium-calmodulin signaling. Steroidogenesis is also aided by synthesis of StAR protein. Estradiol-17β stimulates the formation of the maturation-promoting factor (MPF) complex by cdc25 activation and Myt1 inactivation. LY294002 aids in keeping cdc25 activated by inhibiting calcium-calmodulin induced phosphorylation of Akt which is a negative regulator of mitogen-activated protein kinases. VIH induced activation of Myt1, through protein kinase A (PKA), was inhibited by Rp-cAMP which inhibits adenylate cyclase, thus stabilizing the activated MPF complex. To conclude, the coordinated effect of inhibitors and 5HT accelerates the development of ovary from previtellogenic to matured oocytes, yielding high quality and quantity larvae compared with eyestalk-ablated P. monodon. |
| Databáze: | OpenAIRE |
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