CD14(++) CD16(-) monocytes are the main source of 11β-HSD type 1 after IL-4 stimulation
| Autor: | Macarena Gomez-Lira, Luca Idolazzi, Giulio Bassi, Davide Gatti, Fabio Poli, Vidya Satheesn Kunnathully, Maurizio Rossini, Elisa Zoratti, Silvano Adami, Valentina La Verde, Ombretta Viapiana |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
CD14 medicine.medical_treatment Immunology Stimulation CD16 Biology M2 Peripheral blood mononuclear cell Flow cytometry 03 medical and health sciences 0302 clinical medicine 11β-HSD1 anti-inflammatory glucocorticoids IL-4 monocytes medicine Immunology and Allergy Interleukin 4 Pharmacology medicine.diagnostic_test Cell sorting Molecular biology 030104 developmental biology Cytokine 030215 immunology |
| Popis: | The anti-inflammatory actions of IL-4 are well established through earlier findings. However, the exact mechanism it uses to downregulate the pro-inflammatory cytokine production through monocytes and macrophages is poorly understood. In this study, we examined the effect of IL-4 in the induction of 11β-HSD1 in the two main classes of monocytes, CD14++ CD16- (CD14) and CD14+ CD16+ (CD16). Peripheral Blood Mononuclear Cells (PBMCs) were isolated from 17 healthy donors and were sorted into CD14 and CD16 subpopulations using cell sorting. Effect of IL-4 on 11β-HSD1-enzyme activity was measured in sorted and unsorted monocytes using Homogeneous Time-Resolved Fluorescence (HTRF) and M1/M2 polarization analysis was performed by flow cytometry. Our results indicate that CD14 cells are the major source of 11β-HSD1 enzyme after IL-4 stimulation and that M2 phenotype is not a pre-requisite for its synthesis. |
| Databáze: | OpenAIRE |
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