Loss of the extraembryonic ectoderm inElf5mutants leads to defects in embryonic patterning
| Autor: | Ric Broadhurst, Peter L. Pfeffer, Helen W. Davey, Martyn Donnison, Phil L'Huillier, Angela Beaton |
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| Rok vydání: | 2005 |
| Předmět: |
Mesoderm
animal structures Genotype Ectoderm Biology Polymerase Chain Reaction Mice medicine Animals Molecular Biology Transcription factor In Situ Hybridization Body Patterning DNA Primers Genetics Reverse Transcriptase Polymerase Chain Reaction Trophoblast Embryo Gastrula Embryonic stem cell Trophoblasts Cell biology DNA-Binding Proteins Gastrulation Blotting Southern medicine.anatomical_structure Mutation embryonic structures Endoderm Gene Deletion Transcription Factors Developmental Biology |
| Zdroj: | Development. 132:2299-2308 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.01819 |
| Popis: | The extraembryonic ectoderm (ExE) is essential for mammalian placental formation and survival of the embryo in utero. We have obtained a mouse model lacking the ExE, by targeted deletion of the transcription factor Elf5. Although Elf5 mutant embryos implant and form an ectoplacental cone, no trophoblast stem (TS) cells can be derived, indicating that the absence of ExE is a result of the lack of TS cell maintenance. Embryos without ExE tissue are able to form the anterior visceral endoderm but fail to undergo gastrulation, demonstrating an essential role for the ExE in embryonic patterning during a defined window of development. |
| Databáze: | OpenAIRE |
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