Dynamics of the basement membrane in invasive epithelial clusters in Drosophila

Autor: Caroline Medioni, Stéphane Noselli
Přispěvatelé: Institut de signalisation, biologie du développement et cancer (ISBDC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2005
Předmět:
MESH: Signal Transduction
Embryo
Nonmammalian

MESH: Drosophila
Basement Membrane
MESH: Recombinant Proteins
0302 clinical medicine
Laminin
Cell Movement
Genes
Reporter

Border cells
MESH: Microscopy
Confocal

MESH: Animals
MESH: Basement Membrane
MESH: Cell Movement
[SDV.BDD]Life Sciences [q-bio]/Development Biology
0303 health sciences
Microscopy
Confocal

biology
Cell migration
Protein-Tyrosine Kinases
Recombinant Proteins
Cell biology
medicine.anatomical_structure
Drosophila melanogaster
Transcytosis
MESH: Epithelial Cells
Drosophila
Signal Transduction
Collagen Type IV
MESH: Trans-Activators
Perlecan
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
MESH: Protein-Tyrosine Kinases
MESH: Drosophila melanogaster
03 medical and health sciences
Genetic model
medicine
Animals
Molecular Biology
MESH: Collagen Type IV
030304 developmental biology
Basement membrane
MESH: Genes
Reporter

MESH: Embryo
Nonmammalian

Epithelial Cells
Epithelium
biology.protein
Trans-Activators
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Development (Cambridge, England)
Development (Cambridge, England), Company of Biologists, 2005, 132 (13), pp.3069-77. ⟨10.1242/dev.01886⟩
Development (Cambridge, England), Company of Biologists, 2005, 132, pp.3069-3077
Development (Cambridge, England), 2005, 132, pp.3069-3077
ISSN: 1477-9129
0950-1991
DOI: 10.1242/dev.01886⟩
Popis: The basement membrane (BM) represents a barrier to cell migration, which has to be degraded to promote invasion. However, the role and behaviour of the BM during the development of pre-invasive cells is only poorly understood. Drosophila border cells (BCs) provide an attractive genetic model in which to study the cellular mechanisms underlying the migration of mixed cohorts of epithelial cells. BCs are made of two different epithelial cell types appearing sequentially during oogenesis: the polar cells and the outer BCs. Here, we show that the pre-invasive polar cells undergo an unusual and asymmetrical apical capping with major basement membrane proteins, including the two Drosophila Collagen IV α chains, Laminin A and Perlecan. Capping of polar cells proceeds through a novel, basal-to-apical transcytosis mechanism that involves the small GTPase Drab5. Apical capping is transient and is followed by rapid shedding prior to the initiation of BC migration, suggesting that the apical cap blocks migration. Consistently,non-migratory polar cells remain capped. We further show that JAK/STAT signalling and recruitment of outer BCs are required for correct shedding and migration. The dynamics of the BM represents a marker of migratory BC,revealing a novel developmentally regulated behaviour of BM coupled to epithelial cell invasiveness.
Databáze: OpenAIRE
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