Dynamics of the basement membrane in invasive epithelial clusters in Drosophila
Autor: | Caroline Medioni, Stéphane Noselli |
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Přispěvatelé: | Institut de signalisation, biologie du développement et cancer (ISBDC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) |
Jazyk: | angličtina |
Rok vydání: | 2005 |
Předmět: |
MESH: Signal Transduction
Embryo Nonmammalian MESH: Drosophila Basement Membrane MESH: Recombinant Proteins 0302 clinical medicine Laminin Cell Movement Genes Reporter Border cells MESH: Microscopy Confocal MESH: Animals MESH: Basement Membrane MESH: Cell Movement [SDV.BDD]Life Sciences [q-bio]/Development Biology 0303 health sciences Microscopy Confocal biology Cell migration Protein-Tyrosine Kinases Recombinant Proteins Cell biology medicine.anatomical_structure Drosophila melanogaster Transcytosis MESH: Epithelial Cells Drosophila Signal Transduction Collagen Type IV MESH: Trans-Activators Perlecan [SDV.BC]Life Sciences [q-bio]/Cellular Biology MESH: Protein-Tyrosine Kinases MESH: Drosophila melanogaster 03 medical and health sciences Genetic model medicine Animals Molecular Biology MESH: Collagen Type IV 030304 developmental biology Basement membrane MESH: Genes Reporter MESH: Embryo Nonmammalian Epithelial Cells Epithelium biology.protein Trans-Activators 030217 neurology & neurosurgery Developmental Biology |
Zdroj: | Development (Cambridge, England) Development (Cambridge, England), Company of Biologists, 2005, 132 (13), pp.3069-77. ⟨10.1242/dev.01886⟩ Development (Cambridge, England), Company of Biologists, 2005, 132, pp.3069-3077 Development (Cambridge, England), 2005, 132, pp.3069-3077 |
ISSN: | 1477-9129 0950-1991 |
DOI: | 10.1242/dev.01886⟩ |
Popis: | The basement membrane (BM) represents a barrier to cell migration, which has to be degraded to promote invasion. However, the role and behaviour of the BM during the development of pre-invasive cells is only poorly understood. Drosophila border cells (BCs) provide an attractive genetic model in which to study the cellular mechanisms underlying the migration of mixed cohorts of epithelial cells. BCs are made of two different epithelial cell types appearing sequentially during oogenesis: the polar cells and the outer BCs. Here, we show that the pre-invasive polar cells undergo an unusual and asymmetrical apical capping with major basement membrane proteins, including the two Drosophila Collagen IV α chains, Laminin A and Perlecan. Capping of polar cells proceeds through a novel, basal-to-apical transcytosis mechanism that involves the small GTPase Drab5. Apical capping is transient and is followed by rapid shedding prior to the initiation of BC migration, suggesting that the apical cap blocks migration. Consistently,non-migratory polar cells remain capped. We further show that JAK/STAT signalling and recruitment of outer BCs are required for correct shedding and migration. The dynamics of the BM represents a marker of migratory BC,revealing a novel developmentally regulated behaviour of BM coupled to epithelial cell invasiveness. |
Databáze: | OpenAIRE |
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