New method for production of influenza a virus subtype specific antisera: Employment of precipitate obtained by crossed immunoelectrophoretic techniques as antigen
| Autor: | I.R. Pedersen, A. Hornsleth, C.H. Mordhorst |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Hemagglutination Inhibition Tests
Immunology Fluorescent Antibody Technique Immunoelectrophoresis Biology medicine.disease_cause Immunoenzyme Techniques Antigen Antibody Specificity Influenza A virus medicine Animals Humans Immunology and Allergy Antiserum chemistry.chemical_classification medicine.diagnostic_test Immune Sera Virology Molecular biology chemistry biology.protein Rabbits Antibody Glycoprotein Immunoelectrophoresis Two-Dimensional Neuraminidase |
| Zdroj: | Journal of Immunological Methods. 39:95-110 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(80)90298-7 |
| Popis: | The crossed immunoelectrophoretic technique was employed for the production of subtype-specific influenza A virus antisera. Glycoprotein-containing precipitates were produced by this technique by using detergent solubilized influenza A virus glycoproteins as antigens in the first dimension gel and rabbit antisera against these antigens in the second dimension gel. By cutting these precipitates out of the gel and using them for the immunization of rabbits, antisera were produced which showed the presence of subtype-specific antibodies in haemagglutination inhibition and immunofluorescent antibody tests but no antibodies in neuraminidase inhibition tests. |
| Databáze: | OpenAIRE |
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