A Method Detection Limit for Bacillus anthracis Spores in Water Using an Automated Waterborne Pathogen Concentrator
| Autor: | Robert A LaBudde, Ben Humrighouse, H. D. Alan Lindquist, Vicente J. Gallardo, Adin Pemberton |
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| Rok vydání: | 2015 |
| Předmět: |
Pharmacology
Detection limit Spores Bacterial Chromatography biology Analytical chemistry Ultrafiltration biology.organism_classification Analytical Chemistry Spore Bacillus anthracis Automation Tap water Limit of Detection Reagent Waterborne pathogen Environmental Chemistry Bacillus anthracis - Sterne strain Water Microbiology Agronomy and Crop Science Food Science |
| Zdroj: | Journal of AOAC International. 98(4) |
| ISSN: | 1060-3271 |
| Popis: | The method detection limit (MDL, 99% chance of detecting a positive result in a single replicate), as per the United States Code of Federal Regulations, was determined for a protocol using an ultrafiltration based automated waterborne pathogen concentration device. Bacillus anthracis Sterne strain spores were seeded at low levels into 100 L reagent water samples. Suspect colonies were confirmed through morphological, chemical, and genetic tests. Samples of 100 L (n=14) of reagent water were seeded with five B. anthracis CFUs each. To confirm the estimated detection limit, a second set (n=19) of 100 L reagent water samples were seeded at a higher level (7 CFUs). The second estimate of the MDL could not be pooled with the first, due to significant difference in variance. A third trial (n=7) seeded with 10 CFUs produced an estimate of the MDL that could be pooled with the higher previous estimate. Another trial consisting of eight 100 L samples of tap water were seeded with approximately 7 CFUs. Recovery in these samples was not significantly different from the pooled MDL. Theoretically a concentration of 4.6 spores/100 L would be required for detection 95% of the time, based on a Poisson distribution. The calculated pooled MDL, based on experimental data was approximately 6 B. anthracis CFU/100 L (95% confidence interval 4.8 to 8.4). Detection at this level was achieved in municipal water samples. |
| Databáze: | OpenAIRE |
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