DNA barcoding of native Caucasus herbal plants: potentials and limitations in complex groups and implications for phylogeographic patterns
| Autor: | Silvia Fineschi, Parvin Aghayeva, Salvatore Cozzolino, Valida Alizade, Donata Cafasso, Dilzara N. Aghayeva |
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| Přispěvatelé: | Aghayeva, Parvin, Cozzolino, Salvatore, Cafasso, Donata, Valida Ali-zade, 2, Silvia Fineschi, 1, and Dilzara Aghayeva, 3 |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine barcoding ident Caucasus Azerbaijan QH301-705.5 Lineage (evolution) Asterids accD Barcode 010603 evolutionary biology 01 natural sciences DNA barcoding law.invention 03 medical and health sciences Monophyly law asterids orchids Biology (General) Ecology Evolution Behavior and Systematics Ecology biology native plants biology.organism_classification rpoB floristic surveys Phylogeography 030104 developmental biology Evolutionary biology Identification (biology) ITS barcoding identification Research Article |
| Zdroj: | Biodiversity Data Journal Biodiversity Data Journal, Vol 9, Iss, Pp 1-28 (2021) Biodiversity Data Journal 9: e61333 |
| ISSN: | 1314-2828 |
| Popis: | DNA barcoding has rapidly become a useful complementary tool in floristic investigations particularly for identifying specimens that lack diagnostic characters. Here, we assess the capability of three DNA barcode markers (chloroplast rpoB, accD and nuclear ITS) for correct species assignment in a floristic survey on the Caucasus. We focused on two herbal groups with potential for ornamental applications, namely orchids and asterids. On these two plant groups, we tested whether our selection of barcode markers allows identification of the “barcoding gap” in sequence identity and to distinguish between monophyletic species when employing distance-based methods. All markers successfully amplified most specimens, but we found that the rate of species-level resolution amongst selected markers largely varied in the two plant groups. Overall, for both lineages, plastid markers had a species-level assignment success rate lower than the nuclear ITS marker. The latter confirmed, in orchids, both the existence of a barcoding gap and that all accessions of the same species clustered together in monophyletic groups. Further, it also allowed the detection of a phylogeographic signal.The ITS marker resulted in its being the best performing barcode for asterids; however, none of the three tested markers showed high discriminatory ability. Even if ITS were revealed as the most promising plant barcode marker, we argue that the ability of this barcode for species assignment is strongly dependent on the evolutionary history of the investigated plant lineage. |
| Databáze: | OpenAIRE |
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