Identification and Functional Characterization of PtoMYB055 Involved in the Regulation of the Lignin Biosynthesis Pathway in Populus tomentosa

Autor: Sha Ren, Keming Luo, Yiming Sun, Shenglong Ye, Qiaoyan Tian
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Lignin
01 natural sciences
lcsh:Chemistry
chemistry.chemical_compound
Cell Wall
Gene Expression Regulation
Plant
MYB
lcsh:QH301-705.5
Spectroscopy
Plant Proteins
Chemistry
ectopic deposition
food and beverages
Populus tomentosa
General Medicine
Plants
Genetically Modified
Wood
Up-Regulation
Computer Science Applications
Cell biology
Populus
secondary cell wall
Secondary cell wall
Transcriptional Activation
Phloem
complex mixtures
Article
activator
Catalysis
Inorganic Chemistry
03 medical and health sciences
Xylem
Physical and Theoretical Chemistry
Molecular Biology
Transcription factor
Cell Nucleus
Activator (genetics)
Gene Expression Profiling
fungi
Organic Chemistry
Biosynthetic Pathways
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Transcription Factor Gene
lignin biosynthesis
Transcription Factors
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 14
International Journal of Molecular Sciences, Vol 21, Iss 4857, p 4857 (2020)
ISSN: 1422-0067
DOI: 10.3390/ijms21144857
Popis: Wood, which is mainly composed of lignified secondary cell wall, is the most abundant biomass in woody plants. Previous studies have revealed that R2R3-type MYB transcription factors are important regulators of the formation of the secondary cell wall in vascular plants. In this study, we isolated the R2R3-type MYB transcription factor gene PtoMYB055, which is mainly expressed in xylem and phloem tissue, from Populus tomentosa and demonstrate that PtoMYB055 is a key regulator of lignin biosynthesis. PtoMYB055 as a transcriptional activator is localized to the nucleus. Overexpression of PtoMYB055 upregulates expression of lignin biosynthetic genes in transgenic poplar plants, resulting in ectopic deposition of lignin in phloem tissue and an increase in thickness of the secondary cell wall. In sum, PtoMYB055 is a transcriptional activator that is involved in regulating lignin biosynthesis during the formation of the secondary cell wall in poplar.
Databáze: OpenAIRE
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