Incorporation ofN-propanoylneuraminic acid leads to calcium oscillations in oligodendrocytes upon the application of GABA
| Autor: | Helmut Kettenmann, Werner Reutter, Carsten Ohlemeyer, Rüdiger Horstkorte, Carolin Schmidt |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Glycosylation Glycoconjugate Biochemistry chemistry.chemical_compound Adenosine Triphosphate Structural Biology Neuraminic acid Cells Cultured gamma-Aminobutyric Acid Oligodendrocyte progenitor cell chemistry.chemical_classification Aniline Compounds Glutamate receptor Immunohistochemistry Cell biology Oligodendroglia medicine.anatomical_structure N-Propanoylneuraminic acid Histamine Astrocyte Epinephrine Biophysics Glutamic Acid chemistry.chemical_element O4 Calcium Biology Genetics medicine Animals N-Propanoylmannosamine Calcium Signaling Rats Wistar Molecular Biology Cell Size Calcium metabolism Hexosamines Cell Biology Coculture Techniques Oligodendrocyte Rats Biochemical engineering Animals Newborn Xanthenes chemistry Cell culture Astrocytes Potassium Neuraminic Acids |
| Zdroj: | FEBS Letters. 478:276-280 |
| ISSN: | 0014-5793 |
| Popis: | Sialylation of glycoproteins and glycolipids plays an important role during development, regeneration and pathogenesis. It has been shown that unnatural sialylation within glial cell cultures can have distinct effects on their proliferation and antigenic profiles. These cultures metabolize N-propanoylmannosamine (N-propanoylneuraminic acid precursor=P-NAP), a synthetic non-physiological precursor of neuraminic acid, resulting in the expression of N-propanoylneuraminic acid in glycoconjugates of their cell membranes [Schmidt, C., Stehling, P., Schnitzer, J., Reutter, W. and Horstkorte, R. (1998) J. Biol. Chem. 273, 19146–19152]. To determine whether these biochemically engineered sialic acids influence calcium concentrations in cells of the oligodendrocyte lineage, mixed glial cultures of oligodendrocytes growing on top of an astrocyte monolayer were exposed to glutamate, histamine, adrenaline, γ-aminobutyric acid (GABA), high potassium (high K+) and ATP. Calcium responses in P-NAP-treated oligodendrocytes were determined by confocal microscopy with the calcium indicator fluo-3 AM, and compared with control cultures. We showed that P-NAP differentially modulated the calcium responses of individual oligodendrocytes when GABA was applied. GABA induced calcium oscillations with up to four spikes per min in 60% of oligodendrocytes when treated with P-NAP. |
| Databáze: | OpenAIRE |
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