Change in prostaglandin signaling during sickness syndrome hyperalgesia after ovariectomy in female rats

Autor: I.K. Maba, Aleksander Roberto Zampronio, J.V. Cruz
Rok vydání: 2020
Předmět:
Lipopolysaccharides
medicine.medical_specialty
Lipopolysaccharide
medicine.medical_treatment
Ovariectomy
Intraperitoneal injection
Prostaglandin
Estrous Cycle
Dinoprostone
03 medical and health sciences
Behavioral Neuroscience
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
medicine
Cyclic AMP
Animals
Guanine Nucleotide Exchange Factors
Cyclic adenosine monophosphate
Prostaglandin E2
Rats
Wistar
030304 developmental biology
Illness Behavior
Estrous cycle
0303 health sciences
Sulfonamides
Chemistry
Hydrazones
Isoxazoles
Isoquinolines
Cyclic AMP-Dependent Protein Kinases
Rats
Disease Models
Animal
Endocrinology
Hyperalgesia
Ovariectomized rat
lipids (amino acids
peptides
and proteins)
Female
medicine.symptom
hormones
hormone substitutes
and hormone antagonists
030217 neurology & neurosurgery
medicine.drug
Signal Transduction
Zdroj: Behavioural brain research. 410
ISSN: 1872-7549
Popis: The present study investigated hyperalgesia during sickness syndrome in female rats. Hyperalgesia was induced by an intraperitoneal injection of lipopolysaccharide (LPS) or an intracerebroventricular injection of prostaglandin E2 (PGE2). No differences were found in basal mechanical and thermal thresholds or in LPS-induced hyperalgesia in sham-operated animals in the diestrus or proestrus phase or in ovariectomized (OVX) animals. However, higher levels of PGE2 where found in the cerebrospinal fluid of OVX animals compared to sham-operated females. Intracerebroventricular injection of PGE2 produced rapid mechanical hyperalgesia in sham-operated rats while these responses were observed at later times in OVX animals. The protein kinase A (PKA) inhibitor H-89 reduced mechanical PGE2-induced hyperalgesia in OVX female rats, whereas no effect was observed in sham-operated animals. In contrast, the exchange protein activated by cyclic adenosine monophosphate (cAMP; Epac) inhibitor ESI-09 reduced mechanical PGE2-induced hyperalgesia, whereas no effect was observed in OVX animals. PGE2 also induced thermal hyperalgesia in sham-operated and OVX female rats and a similar effect of ESI-09 was observed. These results suggest that PGE2-induced hyperalgesia that is observed during sickness syndrome has different signaling mechanisms in cycling and OVX female rats involving the activation of the cAMP-Epac or cAMP-PKA pathways, respectively.
Databáze: OpenAIRE
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