Dialysis-Related Genotoxicity: Sister Chromatid Exchanges and DNA Lesions in T and B Lymphocytes of Uremic Patients. Genomic Damage in Patients on Hemodiafiltration
| Autor: | Chiara Caccamo, Manila Criseo, Giuseppe Coppolino, Michele Buemi, Antonio Barillà, Susanna Campo, Lorena Nostro, Francesco Pernice, G. Bonvissuto, Eleonora Crascì, Nadia Belghity, Fulvio Floccari, Adriana Arena, C. Costa |
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| Rok vydání: | 2006 |
| Předmět: |
Male
DNA damage T-Lymphocytes medicine.medical_treatment genome damage comet assay sister chromatid exchange hemodiafiltration oxidative stress Sister chromatid exchange Biology medicine.disease_cause comet assay genome damage medicine Humans oxidative stress Sister chromatids Dialysis Uremia B-Lymphocytes hemodiafiltration Hematology General Medicine Middle Aged Comet assay Nephrology sister chromatid exchange Immunology Kidney Failure Chronic Female Hemodialysis Oxidative stress Genotoxicity DNA Damage |
| Zdroj: | Blood Purification. 24:569-574 |
| ISSN: | 1421-9735 0253-5068 |
| Popis: | Background/Aims: Patients with chronic renal failure show the presence of massive oxidative genome damage but the role played by dialysis is still a controversial issue. The aim of our study was to verify the genomic damage in B- and T-lymphocyte subpopulations of uremic patients after a single hemodiafiltration session. Methods: We enrolled 30 patients on maintenance acetate-free biofiltration and 25 age-matched healthy volunteers and studied chromosomal alterations. Results: Our data show that the basal levels of DNA damage, the number of sister chromatid exchanges and basal high-frequency cells levels are significantly higher in patients on hemodiafiltration than in controls and in T lymphocytes than in B cells. Conclusions: These findings suggest that hemodialytic treatment could represent a potential source of damage, maybe through the oxidative action of the extracorporeal circuit components, which might explain the well-known T-specific immunodeficiency correlated with uremia. |
| Databáze: | OpenAIRE |
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