Validation of a score tool for measurement of histological severity in juvenile dermatomyositis and association with clinical severity of disease
| Autor: | Hemlata, Varsani, Susan C, Charman, Charles K, Li, Suely K N, Marie, Anthony A, Amato, Brenda, Banwell, Kevin E, Bove, Andrea M, Corse, Alison M, Emslie-Smith, Thomas S, Jacques, Ingrid E, Lundberg, Carlo, Minetti, Inger, Nennesmo, Elisabeth J, Rushing, Adriana M E, Sallum, Caroline, Sewry, Clarissa A, Pilkington, Janice L, Holton, Lucy R, Wedderburn, Linda, Suffiel |
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| Přispěvatelé: | University of Zurich, Wedderburn, Lucy R |
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty CD3 Complex Visual analogue scale Intraclass correlation 2745 Rheumatology Biopsy Immunology 10208 Institute of Neuropathology Antigens Differentiation Myelomonocytic 610 Medicine & health Myosins Biceps Severity of Illness Index General Biochemistry Genetics and Molecular Biology Dermatomyositis Quadriceps Muscle Rheumatology 1300 General Biochemistry Genetics and Molecular Biology Antigens CD Internal medicine Severity of illness medicine Immunology and Allergy Humans Child Muscle Skeletal Disease Activity Basic and Translational Research Juvenile dermatomyositis Inflammation 2403 Immunology Muscle biopsy medicine.diagnostic_test business.industry Histocompatibility Antigens Class I Reproducibility of Results medicine.disease Immunohistochemistry Child Preschool 2723 Immunology and Allergy Physical therapy 570 Life sciences biology Female business |
| Zdroj: | Annals of the Rheumatic Diseases |
| ISSN: | 1468-2060 |
| Popis: | ObjectivesTo study muscle biopsy tissue from patients with juvenile dermatomyositis (JDM) in order to test the reliability of a score tool designed to quantify the severity of histological abnormalities when applied to biceps humeri in addition to quadriceps femoris. Additionally, to evaluate whether elements of the tool correlate with clinical measures of disease severity.Methods55 patients with JDM with muscle biopsy tissue and clinical data available were included. Biopsy samples (33 quadriceps, 22 biceps) were prepared and stained using standardised protocols. A Latin square design was used by the International Juvenile Dermatomyositis Biopsy Consensus Group to score cases using our previously published score tool. Reliability was assessed by intraclass correlation coefficient (ICC) and scorer agreement (α) by assessing variation in scorers’ ratings. Scores from the most reliable tool items correlated with clinical measures of disease activity at the time of biopsy.ResultsInter- and intraobserver agreement was good or high for many tool items, including overall assessment of severity using a Visual Analogue Scale. The tool functioned equally well on biceps and quadriceps samples. A modified tool using the most reliable score items showed good correlation with measures of disease activity.ConclusionsThe JDM biopsy score tool has high inter- and intraobserver agreement and can be used on both biceps and quadriceps muscle tissue. Importantly, the modified tool correlates well with clinical measures of disease activity. We propose that standardised assessment of muscle biopsy tissue should be considered in diagnostic investigation and clinical trials in JDM. |
| Databáze: | OpenAIRE |
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