Expression Profiles of p53-, p16INK4a-, and Telomere-Regulating Genes in Replicative Senescent Primary Human, Mouse, and Chicken Fibroblast Cells
| Autor: | Seungkwon You, Byung-Whi Kong, Shelly A. Christman, Hyunggee Kim, Linda K. Foster, Douglas N. Foster, Farris James A |
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| Rok vydání: | 2002 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
Senescence Telomerase Transcription Genetic Cell division Cell Cell Cycle Proteins Biology Retinoblastoma Protein Mice Cyclins Proto-Oncogene Proteins medicine Animals Humans Fibroblast Cells Cultured Cellular Senescence Cyclin-Dependent Kinase Inhibitor p16 Regulation of gene expression Mice Inbred BALB C Gene Expression Profiling Nuclear Proteins Proto-Oncogene Proteins c-mdm2 Cell Biology Fibroblasts Telomere Embryonic stem cell Molecular biology E2F Transcription Factors DNA-Binding Proteins medicine.anatomical_structure Gene Expression Regulation embryonic structures Tumor Suppressor Protein p53 Chickens Cell Division HeLa Cells Transcription Factors |
| Zdroj: | Experimental Cell Research. 272:199-208 |
| ISSN: | 0014-4827 |
| DOI: | 10.1006/excr.2001.5420 |
| Popis: | Replicative senescence is known to be an intrinsic mechanism in determining the finite life span of in vitro cultured cells. Since this process is recognized as an evolutionarily conserved mechanism from yeast to mammalian cells, we compared the senescence-associated genetic alterations in the p53, p16(INK4a), and telomere regulatory pathways using replicative senescent human, mouse, and chicken fibroblast cells. Normal human diploid fibroblast (HDF; WI38) and chicken embryonic fibroblast (CEF) cells were shown to have a more extended in vitro proliferative potential than their mouse embryonic fibroblast (MEF) counterpart. In contrast to the HDF and CEF cells, MEF cells were shown to express telomerase mRNA and maintain telomerase activity throughout their in vitro life span. Functional p53 activity was shown to increase in the replicative senescent HDF and CEF cells, but not in replicative senescent MEF cells. On the other hand, there was a gradual elevation of p16(INK4a) expression with increased cell passages which reached a maximum in replicative senescent MEF cells. Taken together, the present study demonstrates that the p53, p16(INK4a), and telomere regulatory functions may be differentially regulated during replicative senescence in human, mouse, and chicken fibroblast cells. |
| Databáze: | OpenAIRE |
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