Evidence that CTP:choline-phosphate cytidylyltransferase is regulated at a pretranslational level in rat liver after partial hepatectomy
| Autor: | Joseph J. Batenburg, Gabriel B. Kalmar, Lambert M.G. van Golde, Lilian B. M. Tijburg, Rosemary B. Cornell, W.J. Vaartjes, Martin Houweling |
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| Rok vydání: | 1993 |
| Předmět: |
Male
medicine.medical_specialty Cytidylyltransferase Biology Biochemistry Phosphatidylcholine Biosynthesis Choline-phosphate cytidylyltransferase chemistry.chemical_compound Cytosol Internal medicine Phosphatidylcholine medicine Animals Hepatectomy Choline Choline-Phosphate Cytidylyltransferase RNA Messenger Northern blot Rats Wistar Methionine Nucleotidyltransferases Cytidylyltransferase activity Liver Regeneration Rats Isoenzymes Endocrinology Gene Expression Regulation Liver chemistry Phosphatidylcholines |
| Zdroj: | European Journal of Biochemistry. 214:927-933 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1993.tb17996.x |
| Popis: | Regulation of CTP:choline-phosphate cytidylyltransferase activity was studied in regenerating rat liver. The formation of phosphatidylcholine from [14C]choline in hepatocytes isolated from regenerating liver at 22 h after surgery was increased 1.9-fold when compared with hepatocytes from sham-operated animals. This effect was accompanied by a 1.4-fold increase in cytosolic cytidylyltransferase activity as well as by a 1.5-fold increase in the amount of immunoreactive cytidylyltransferase protein, and a 1.7-fold increase in [35S]methionine incorporation into cytidylyltransferase protein. Northern blot analysis of cytidylyltransferase mRNA showed two signals at 1.5 and 5.0 kb. Partial hepatectomy caused a significant 2–3-fold increase in the 1.5-kb and 5.0-kb messengers at 12 h after surgery. During the next 10 h after partial hepatectomy cytidylyltransferase mRNA levels slightly decreased, although they were still elevated in comparison with sham-operated rats 20–22 h after surgery. In contrast to the elevated cytidylyltransferase mRNA levels, the amount of acetyl-CoA carboxylase mRNA did not increase between 12 and 22 h after surgery, which is in line with the unchanged activity of this enzyme. In conclusion, our data demonstrate that in regenerating liver phosphatidylcholine biosynthesis and cytidylyltransferase activity are regulated at a pretranslational level. |
| Databáze: | OpenAIRE |
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