Transfer of IVF-contaminated blastocysts with removal of the zona pellucida resulted in live births
| Autor: | Yimin Shu, Robert N. Taylor, David Prokai, E.B. Johnston-MacAnanny, Sarah L. Berga |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult animal structures Pregnancy Rate medicine.medical_treatment Fertilization in Vitro Biology Andrology 03 medical and health sciences 0302 clinical medicine Pregnancy Genetics medicine Humans Blastocyst Sperm Injections Intracytoplasmic Zona pellucida Genetics (clinical) Zona Pellucida 030219 obstetrics & reproductive medicine In vitro fertilisation Obstetrics and Gynecology Embryo Embryo culture General Medicine Contamination Embryo Transfer Vitrification Embryo transfer Embryo Biology Pregnancy rate 030104 developmental biology medicine.anatomical_structure Reproductive Medicine embryonic structures Oocytes Female Live Birth Developmental Biology |
| Zdroj: | Journal of assisted reproduction and genetics. 33(10) |
| ISSN: | 1573-7330 |
| Popis: | Bacterial contamination may cause loss or damage to cultured oocytes or embryos, resulting in cancelation or delaying of a fresh embryo transfer. While live births have been reported following the transfer of embryos contaminated with yeast, very little information is available on how to handle embryos with bacterial contamination. We report two cases of successful pregnancy in patients with bacterial contamination of embryo culture dishes.We retrospectively reviewed 878 oocyte retrievals performed between January 2011 and December 2014. Bacterial contamination was recorded in two split IVF/ICSI cases, where contamination occurred in embryo culture drops containing embryos from conventional insemination but not from ICSI on day 3.To minimize the adverse effects of bacterial contamination on transfer outcomes, we removed the zona pellucida of contaminated frozen blastocysts and successfully obtained clinical pregnancies following transfer of zona-free blastocysts that were previously contaminated during IVF culture.Removal of the zona pellucida is an appropriate approach to handle blastocysts contaminated with bacteria during in vitro culture. |
| Databáze: | OpenAIRE |
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