The Rhizobium sp. BR816 nodD 3 gene is regulated by a transcriptional regulator of the AraC/XylS family
| Autor: | Jozef Vanderleyden, K. M. Vlassak, P. De Wilde, Carla Snoeck, P van Rhijn, Ellen Luyten |
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| Rok vydání: | 1998 |
| Předmět: |
Transcriptional Activation
Recombinant Fusion Proteins AraC Transcription Factor Molecular Sequence Data Restriction Mapping Regulator Sigma Factor Microbiology Open Reading Frames Plasmid Bacterial Proteins Genetics Transcriptional regulation Molecular Biology Gene Glucuronidase Regulation of gene expression Plants Medicinal biology food and beverages Fabaceae DNA-Directed RNA Polymerases Gene Expression Regulation Bacterial biology.organism_classification DNA-Binding Proteins Repressor Proteins Complementation Genes Bacterial Trans-Activators Rhizobium RNA Polymerase Sigma 54 Transcription Factors Bradyrhizobium japonicum |
| Zdroj: | Molecular and General Genetics MGG. 258:558-561 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/s004380050768 |
| Popis: | Rhizobium sp. BR816 contains four nodD alleles of which nodD3 is the most important transcriptional regulator for nodulation of Phaseolus vulgaris. Upstream of nodD3 an open reading frame, orf816, was identified. The deduced ORF816 protein shows homology with transcriptional regulators of the AraC/XylS family. The DNA binding domain and the consensus motif, characteristic of the C-terminal region of the members of this family of transcriptional regulators, are present in the deduced ORF816 protein. Activation of nodA gene expression and nodulation of P. vulgaris by Rhizobium sp. NGR234nodD1::omega (Nod-) complemented with the Rhizobium sp. BR816 nodD3 gene were significantly increased in the presence of orf816. This increased nodulation and nod gene induction are mediated through positive regulation of nodD3 expression by ORF816. Expression of orf816 itself is partially RpoN dependent. The role of this transcriptional regulator in the complex cascade regulation of the Rhizobium sp. nodD3 gene is discussed. |
| Databáze: | OpenAIRE |
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