Isolation and characterization of collagen from the skin of Brama australis
| Autor: | Malgorzata Skorupska, Justyna Kozlowska, Alina Sionkowska, Marta Michalska |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Protein Denaturation
Wet weight Biochemistry Collagen Type I Amino acid analysis Structural Biology Spectroscopy Fourier Transform Infrared Polymer chemistry Animals Denaturation (biochemistry) Amino Acids Molecular Biology Skin Fish skin biology Chemistry Fishes Temperature General Medicine biology.organism_classification Molecular biology Brama australis Electrophoresis Fish Electrophoresis Polyacrylamide Gel Collagen Type I collagen |
| Zdroj: | International Journal of Biological Macromolecules. 80:605-609 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2015.07.032 |
| Popis: | Collagen was extracted from the skin of Brama australis , the fish from warm-water sea. The yield of collagen from skin of B. australis was about 1.5% on a wet weight basis of raw material. The isolated protein was confirmed as collagen by different physico-chemical techniques such as: FTIR, SDS-PAGE, and amino acid analysis. The denaturation temperature ( T d ) of obtained collagen was found to be 24 °C, what is promising as an advantage for cosmetic application. According to the electrophoretic pattern, collagen consisted of two different α-chains (α1 and α2) and was classified as type I collagen. Although T d of obtained collagen is higher than 20 °C it is still far from T d of mammalian collagen. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect