Fabrication of nanostructures of polyethylene glycol for applications to protein adsorption and cell adhesion
| Autor: | Deok Ho Kim, Byungkyu Kim, Sangho Lee, Ali Khademhosseini, Pilnam Kim, Robert Langer, Seung Kyu Choi, Kahp-Yang Suh |
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| Rok vydání: | 2005 |
| Předmět: |
Materials science
Nanostructure Mechanical Engineering technology industry and agriculture Bioengineering Self-assembled monolayer Nanotechnology macromolecular substances General Chemistry Polyethylene glycol Adhesion Soft lithography Contact angle chemistry.chemical_compound Chemical engineering chemistry Mechanics of Materials PEG ratio General Materials Science Electrical and Electronic Engineering Protein adsorption |
| Zdroj: | Nanotechnology. 16:2420-2426 |
| ISSN: | 1361-6528 0957-4484 |
| Popis: | A simple method was developed to fabricate polyethylene glycol (PEG) nanostructures using capillary lithography mediated by ultraviolet (UV) exposure. Acrylate-containing PEG monomers, such as PEG dimethacrylate (PEG-DMA, MW = 330), were photo-cross-linked under UV exposure to generate patterned structures. In comparison to unpatterned PEG films, hydrophobicity of PEG nanostructure modified surfaces was significantly enhanced. This could be attributed to trapped air in the nanostructures as supported by water contact angle measurements. Proteins (fibronectin, immunoglobulin, and albumin) and cells (fibroblasts and P19 EC cells) were examined on the modified surfaces to test for the level of protein adsorption and cell adhesion. It was found that proteins and cells preferred to adhere on nanostructured PEG surfaces in comparison to unpatterned PEG films; however, this level of adhesion was significantly lower than that of glass controls. These results suggest that capillary lithography can be used to fabricate PEG nanostructures capable of modifying protein and cell adhesive properties of surfaces. |
| Databáze: | OpenAIRE |
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