Physical and genetic map of theListeria monocytogenesEGD serotype 1/2a chromosome
| Autor: | Eugen Domann, Trinad Chakraborty, Ulrich von Both, Abdelhak Darbouche, Sonja Otten |
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| Rok vydání: | 1999 |
| Předmět: |
Restriction Mapping
Virulence medicine.disease_cause Microbiology Genome Gene mapping Listeria monocytogenes Genetics Pulsed-field gel electrophoresis medicine Serotyping Molecular Biology biology Chromosome Mapping Chromosome Chromosomes Bacterial Physical Chromosome Mapping biology.organism_classification Electrophoresis Gel Pulsed-Field Restriction site Genes Bacterial Listeria |
| Zdroj: | FEMS Microbiology Letters. 175:281-289 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.1999.tb13632.x |
| Popis: | Listeria monocytogenes is a facultative intracellular pathogen responsible for both invasive and non-invasive food-borne illness in animals and humans. In this study, macrorestriction analysis following pulsed-field gel electrophoresis was used to show that Listeria monocytogenes serovar 1/2a strain EGD has a single chromosome containing eight NotI fragments of 1100, 850, 365, 320, 275, 40, 30 and 20 kb in size and 11 AscI fragments of 860, 470, 410, 360, 320, 250, 110, 80, 50, 30 and 20 kb. The total genome therefore comprises 3000 +/- 50 kb. The creation of a physical and genetic map of the Listeria genome was achieved by generating NotI linking clones and their use in subsequent hybridisation analysis. Using isogenic mutants harbouring additional artificial NotI restriction sites, we were able to precisely map the positions of all currently known virulence genes on the chromosome. |
| Databáze: | OpenAIRE |
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