Synovial fluid is a site of citrullination of autoantigens in inflammatory arthritis
| Autor: | Robin Wait, Ngee Han Lim, Vivianne Malmström, Karin Lundberg, Tore Saxne, Albert J.W. Zendman, Patrick J Venables, Natalia Wegner, Andrew J Kinloch |
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| Rok vydání: | 2008 |
| Předmět: |
Hydrolases
Inflammatory arthritis Immunology Arthritis Protein citrullination Autoantigens Peptides Cyclic Arthritis Rheumatoid Rheumatology Antigen Protein-Arginine Deiminase Type 4 Osteoarthritis Spondylarthritis Synovial Fluid Biomarkers Tumor Immunology and Allergy Medicine Synovial fluid Humans Pharmacology (medical) Gel electrophoresis business.industry Tumor Suppressor Proteins Citrullination medicine.disease DNA-Binding Proteins Rheumatoid arthritis Case-Control Studies Phosphopyruvate Hydratase Protein-Arginine Deiminases Citrulline business |
| Zdroj: | Arthritis and rheumatism. 58(8) |
| ISSN: | 0004-3591 |
| Popis: | OBJECTIVE: To examine synovial fluid as a site for generating citrullinated antigens, including the candidate autoantigen citrullinated alpha-enolase, in rheumatoid arthritis (RA). METHODS: Synovial fluid was obtained from 20 patients with RA, 20 patients with spondylarthritides (SpA), and 20 patients with osteoarthritis (OA). Samples were resolved using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by staining with Coomassie blue and immunoblotting for citrullinated proteins, alpha-enolase, and the deiminating enzymes peptidylarginine deiminase type 2 (PAD-2) and PAD-4. Proteins from an RA synovial fluid sample were separated by 2-dimensional electrophoresis, and each protein was identified by immunoblotting and mass spectrometry. Antibodies to citrullinated alpha-enolase peptide 1 (CEP-1) and cyclic citrullinated peptide 2 were measured by enzyme-linked immunosorbent assay. RESULTS: Citrullinated polypeptides were detected in the synovial fluid from patients with RA and patients with SpA, but not in OA samples. Alpha-enolase was detected in all of the samples, with mean levels of 6.4 ng/microl in RA samples, 4.3 ng/microl in SpA samples, and |
| Databáze: | OpenAIRE |
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