Asxl2−/− Mice Exhibit De Novo Cardiomyocyte Production during Adulthood
| Autor: | Hsiao-Lei Lai, David L. Geenen, Zane Deliu, Rachel Brunner, Elan Melman, Q. Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
chromatin factor proliferation Population Vimentin cardiomyocyte heart 030204 cardiovascular system & hematology 03 medical and health sciences 0302 clinical medicine Internal medicine cardiogenic medicine regeneration epigenetic Epigenetics Myocardial infarction education Molecular Biology lcsh:QH301-705.5 030304 developmental biology 0303 health sciences education.field_of_study biology Regeneration (biology) Wild type Cell Biology medicine.disease 3. Good health Chromatin Endocrinology lcsh:Biology (General) Heart failure biology.protein Developmental Biology |
| Zdroj: | Journal of Developmental Biology, Vol 4, Iss 4, p 32 (2016) Journal of Developmental Biology; Volume 4; Issue 4; Pages: 32 |
| ISSN: | 2221-3759 |
| Popis: | Heart attacks affect more than seven million people worldwide each year. A heart attack, or myocardial infarction, may result in the death of a billion cardiomyocytes within hours. The adult mammalian heart does not have an effective mechanism to replace lost cardiomyocytes. Instead, lost muscle is replaced with scar tissue, which decreases blood pumping ability and leads to heart failure over time. Here, we report that the loss of the chromatin factor ASXL2 results in spontaneous proliferation and cardiogenic differentiation of a subset of interstitial non-cardiomyocytes. The adult Asxl2−/− heart displays spontaneous overgrowth without cardiomyocyte hypertrophy. Thymidine analog labeling and Ki67 staining of 12-week-old hearts revealed 3- and 5-fold increases of proliferation rate for vimentin+ non-cardiomyocytes in Asxl2−/− over age- and sex-matched wildtype controls, respectively. Approximately 10% of proliferating non-cardiomyocytes in the Asxl2−/− heart express the cardiogenic marker NKX2-5, a frequency that is ~7-fold higher than that observed in the wildtype. EdU lineage tracing experiments showed that ~6% of pulsed-labeled non-cardiomyocytes in Asxl2−/− hearts differentiate into mature cardiomyocytes after a four-week chase, a phenomenon not observed for similarly pulse-chased wildtype controls. Taken together, these data indicate de novo cardiomyocyte production in the Asxl2−/− heart due to activation of a population of proliferative cardiogenic non-cardiomyocytes. Our study suggests the existence of an epigenetic barrier to cardiogenicity in the adult heart and raises the intriguing possibility of unlocking regenerative potential via transient modulation of epigenetic activity. |
| Databáze: | OpenAIRE |
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