Regulation of Stearoyl Coenzyme A Desaturase 1 Gene Promoter in Bovine Mammary Cells
| Autor: | Federico Infascelli, Nicoletta Murru, O. di Martino, Viola Calabrò, Monica Isabella Cutrignelli, Annaelena Troiano, Laura Addi, R. Tudisco, Andrea Maria Guarino, Serena Calabrò |
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| Přispěvatelé: | DI MARTINO, Orsola, Troiano, Annaelena, Addi, Laura, Guarino, Andrea, Calabro', Serena, Tudisco, Raffaella, Murru, Nicoletta, Cutrignelli, MONICA ISABELLA, Infascelli, Federico, Calabro', Viola |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Bovine mammary cell
Molecular Sequence Data Bioengineering Biology Cell Line chemistry.chemical_compound Mammary Glands Animal Animals Insulin Regulation of gene expression Luciferase Promoter Regions Genetic Transcription factor chemistry.chemical_classification Reporter gene Base Sequence Stearoyl coenzyme a desaturase Fatty Acids Fatty acid Promoter Molecular biology Stearoyl-CoA Desaturase Oleic acid CCAAT-Binding Factor Gene Expression Regulation chemistry Biochemistry Cattle Female lipids (amino acids peptides and proteins) Animal Science and Zoology Oleic Acid Biotechnology |
| Popis: | Stearoyl-Coenzyme A desaturase 1 (SCD1) belongs to the fatty acid family of desaturases. In lactating ruminants, the SCD1 protein is highly expressed in the mammary gland and is relevant for the fatty acid composition of milk and dairy products. Bovine mammary epithelial cells (BME-UV1), cultured in vitro, have been proposed as a model to reproduce the biology of the mammary gland. The present study was designed to investigate the responsiveness of bovine SCD1 promoter to serum, insulin, oleic acid, and NFY transcription factor in BME-UV1 cells. A luciferase-based reporter assay was used to monitor the transcriptional activity of the SCD1 promoter region in BME-UV1 cells treated or not with insulin and/or oleic acid. The level of endogenous SCD1 mRNA was evaluated by Real time PCR. Insulin (20 ng/mL) induced a 2.0 to 2.5-fold increase of SCD1 promoter activity. Additionally, the effect of insulin was inhibited by oleic acid, serum components, and NFY enforced expression. Serum and NFY showed no synergistic or additive effect on SCD1 promoter activity suggesting that they repress SCD1 transcription through the same responsive element. |
| Databáze: | OpenAIRE |
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