Using PCR to Target Misconceptions about Gene Expression
| Autor: | Dina L. Newman, Leslie Kate Wright |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
QH301-705.5
Cell education Biology Bioinformatics undergraduate biology General Biochemistry Genetics and Molecular Biology Education Green fluorescent protein chemistry.chemical_compound Plasmid Gene expression medicine Biology (General) Gene lcsh:QH301-705.5 Organism Genetics Messenger RNA lcsh:LC8-6691 General Immunology and Microbiology lcsh:Special aspects of education LC8-6691 DNA Special aspects of education medicine.anatomical_structure PCR chemistry lcsh:Biology (General) gene expression Curriculum General Agricultural and Biological Sciences |
| Zdroj: | Journal of Microbiology & Biology Education, Vol 14, Iss 1, Pp 93-100 (2013) Journal of Microbiology & Biology Education, Vol 14, Iss 1 (2013) Journal of Microbiology & Biology Education : JMBE |
| ISSN: | 1935-7877 1935-7885 |
| DOI: | 10.1128/jmbe.v14i1.539 |
| Popis: | We present a PCR-based laboratory exercise that can be used with first- or second-year biology students to help overcome common misconceptions about gene expression. Biology students typically do not have a clear understanding of the difference between genes (DNA) and gene expression (mRNA/protein) and often believe that genes exist in an organism or cell only when they are expressed. This laboratory exercise allows students to carry out a PCR-based experiment designed to challenge their misunderstanding of the difference between genes and gene expression. Students first transform E. coli with an inducible GFP gene containing plasmid and observe induced and un-induced colonies. The following exercise creates cognitive dissonance when actual PCR results contradict their initial (incorrect) predictions of the presence of the GFP gene in transformed cells. Field testing of this laboratory exercise resulted in learning gains on both knowledge and application questions on concepts related to genes and gene expression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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