IL-17A–producing resident memory γδ T cells orchestrate the innate immune response to secondary oral Listeria monocytogenes infection
| Autor: | Leo Lefrançois, Quynh-Mai Pham, Pablo A. Romagnoli, Kamal M. Khanna, Brian S. Sheridan |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Receptors CXCR3 Population Mice Transgenic Biology CXCR3 Microbiology 03 medical and health sciences Chemokine receptor 0302 clinical medicine Immune system Intestinal mucosa Cell Movement T-Lymphocyte Subsets medicine Animals Mesenteric lymph nodes Listeriosis Mesentery Myeloid Cells Intestinal Mucosa education Mice Inbred BALB C education.field_of_study Multidisciplinary Innate immune system Interleukin-17 Receptors Antigen T-Cell gamma-delta Biological Sciences Listeria monocytogenes Intestinal epithelium Immunity Innate 030104 developmental biology medicine.anatomical_structure Immunology Female Lymph Nodes Immunologic Memory 030215 immunology |
| Zdroj: | Proceedings of the National Academy of Sciences. 113:8502-8507 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Memory γδ T cells are important for the clearance of Listeria monocytogenes infection in the intestinal mucosa. However, the mechanisms by which memory γδ T cells provide protection against secondary oral infection are poorly understood. Here we used a recombinant strain of L. monocytogenes that efficiently invades the intestinal epithelium to show that Vγ4+ memory γδ T cells represent a resident memory (Trm) population in the mesenteric lymph nodes (MLNs). The γδ Trm exhibited a remarkably static pattern of migration that radically changed following secondary oral L. monocytogenes infection. The γδ Trms produced IL-17A early after rechallenge and formed organized clusters with myeloid cells surrounding L. monocytogenes replication foci only after a secondary oral infection. Antibody blocking studies showed that in addition to IL-17A, the chemokine receptor C-X-C chemokine receptor 3 (CXCR3) is also important to enable the local redistribution of γδ Trm cells and myeloid cells specifically near the sites of L. monocytogenes replication within the MLN to restrict bacterial growth and spread. Our findings support a role for γδ Trms in orchestrating protective immune responses against intestinal pathogens. |
| Databáze: | OpenAIRE |
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