Model plants for studying the interaction between Methylobacterium mesophilicum and Xylella fastidiosa
| Autor: | Fernando Dini Andreote, Jan Dirk van Elsas, Leonard S. van Overbeek, Paulo Teixeira Lacava, Walter Maccheroni, João Lúcio de Azevedo, Welington Luiz Araújo, C. S. Gai |
|---|---|
| Přispěvatelé: | Van Elsas lab |
| Rok vydání: | 2006 |
| Předmět: |
Citrus
DIVERSITY Xylella Plant Roots Polymerase Chain Reaction Applied Microbiology and Biotechnology RNA Ribosomal 16S DGGE biology RHIZOSPHERE food and beverages Agriculture General Medicine ENDOPHYTIC BACTERIA BRAZIL RNA Bacterial Methylobacterium brazil gradient gel-electrophoresis POPULATIONS Endophytic bacteria Catharanthus EXPERIMENTAL HOST Immunology CULTURES CITRUS PLANTS Microbiology diversity Bacterial genetics resistance citrus plants GRADIENT GEL-ELECTROPHORESIS Tobacco Botany Genetics model plants endophytic Molecular Biology DNA Primers Plant Diseases Biointeracties and Plant Health fungi populations biology.organism_classification cultures Methylobacterium mesophilicum endophytic bacteria Seedlings Biofilms PRI Biointeractions en Plantgezondheid experimental host Xylella fastidiosa rhizosphere RESISTANCE |
| Zdroj: | Canadian Journal of Microbiology, 52(5), 419-426 Canadian Journal of Microbiology 52 (2006) 5 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/w05-142 |
| Popis: | Over the last few years, endophytic bacterial communities associated with citrus have been studied as key components interacting with Xylella fastidiosa. In this study, we investigated the possible interaction between the citrus endophyte Methylobacterium mesophilicum SR1.6/6 and X. fastidiosa in model plants such as Catharanthus roseus (Madagaskar periwinkle) and Nicotiana clevelandii (Clevelands tobacco). The aim of this study was to establish the fate of M. mesophilicum SR1.6/6 after inoculation of C. roseus and N. clevelandii plants, using PCR–DGGE (polymerase chain reaction – denaturing gradient gel electrophoresis) and plating techniques. Shifts in the indigenous endophytic bacterial communities were observed in plants inoculated with strain SR1.6/6, using specific primers targeting α- and β-Proteobacteria. Cells of strain SR1.6/6 were observed in a biofilm structure on the root and hypocotyl surfaces of in vitro seedlings inoculated with M. mesophilicum SR1.6/6. This emphasizes the importance of these tissues as main points of entrance for this organism. The results showed that C. roseus and N. clevelandii could be used as model plants to study the interaction between M. mesophilicum and X. fastidiosa.Key words: endophytic, Methylobacterium, model plants, DGGE. |
| Databáze: | OpenAIRE |
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