Cellular mechanisms underlying carbon monoxide stimulated anion secretion in rat epididymal epithelium
| Autor: | Lei Peng, Dong-Dong Gao, Yi-Lin Zhang, Jia-Wen Xu, Li-Jiao Ke, Wen-Liang Zhou, Yun-Xin Zhu, Jian-Bang Xu, Zhuo-Er Qiu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Cancer Research Physiology Clinical Biochemistry Cystic Fibrosis Transmembrane Conductance Regulator Endogeny 030204 cardiovascular system & hematology Biochemistry Epithelium Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Chloride Channels Organometallic Compounds medicine Animals Secretion Ion transporter Epididymis Carbon Monoxide Ion Transport ATP synthase biology Ussing chamber Chemistry Cell biology Heme oxygenase 030104 developmental biology medicine.anatomical_structure Heme Oxygenase (Decyclizing) biology.protein Intracellular |
| Zdroj: | Nitric Oxide. :30-37 |
| ISSN: | 1089-8603 |
| Popis: | Epididymal epithelium possesses active ion transport properties conducive to the maintenance of appropriate epididymal intraluminal microenvironment. The endogenous gasotransmitter carbon monoxide (CO) regulates numerous cellular processes including water and electrolyte transport in various epithelia. However, the functional role of CO in epididymal epithelium is still elusive. This study aims to explore the potential regulatory effect of CO on transepithelial ion transport in rat epididymis. Using qPCR technique, we verified that endogenous CO synthase heme oxygenase 1 was expressed in rat caput, corpus, and cauda epididymis. In addition, endogenous CO was detected in rat cauda epididymis. Ussing chamber experiments showed that CORM-2, a CO donor, induced an increase of the short-circuit current (ISC) in a concentration-dependent manner in rat cauda epididymal epithelium. The ISC response could be abrogated by removing the ambient Cl- or HCO3-. Interfering with the cAMP signaling pathway or blocking cystic fibrosis transmembrane regulator (CFTR) partially suppressed the CO-stimulated ISC response. Moreover, the CO-evoked ISC response was significantly attenuated by blocking Ca2+-activated Cl- channel (CaCC) or chelating intracellular Ca2+. Elevation of intracellular Ca2+ level was also observed after CO stimulation in rat cauda epididymal epithelial cells. Collectively, this study demonstrated that CO stimulated anion secretion via activation of CFTR and CaCC in rat cauda epididymal epithelium, which might contribute to the formation of the appropriate microenvironment essential for sperm storage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect