Sequence and expression analysis of a cAMP-responsive gene regulated during late development of Dictyostelium discoideum
| Autor: | D. P. Ramji, B. D. Hames, J. T. J. Donovan |
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| Rok vydání: | 1991 |
| Předmět: |
Base Composition
Base Sequence Transcription Genetic biology Molecular Sequence Data Restriction Mapping Primary transcript biology.organism_classification TATA Box Microbiology Molecular biology Dictyostelium Primer extension Dictyostelium discoideum Blotting Southern Transcription (biology) Regulatory sequence Gene Expression Regulation Fungal Gene expression Cyclic AMP DNA Fungal Promoter Regions Genetic Molecular Biology Gene |
| Zdroj: | Molecular Microbiology. 5:427-432 |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1111/j.1365-2958.1991.tb02125.x |
| Popis: | Summary The 7E gene is expressed late in normal development of Dictyostelium discoideum after pseudoplasmodium formation. After disaggregation of the developing cells, transcription of this gene depends entirely on exogenous 3′5′ cyclic AMP (cAMP). The 5′ flanking region of the 7E gene contains two TATA box-oligo (dT) promoter motifs but analysis of 7E gene expression by primer extension shows only a single primary transcript with transcription initiating immediately after the most proximal promoter motif during development or in disaggregated cells in the presence of exogenous cAMP. Four C-rich sequence lie within 350 bp upstream of the cap site, analogus to the upstream elements implicated in the cAMP regulation of several other Dictyostelium genes expressed in development. |
| Databáze: | OpenAIRE |
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