The fission yeast homologue of Glel is essential for growth and involved in mRNA export
| Autor: | Hyun Jin Cho, Jin Ho Yoon, Jin-Ah Bae, DongGeRaMi Moon |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Cell Nucleus
Messenger RNA biology Intron Gene Expression Nuclear Proteins RNA Fungal General Medicine biology.organism_classification Applied Microbiology and Biotechnology Microbiology Yeast RNA Transport Biochemistry Schizosaccharomyces pombe Gene expression Schizosaccharomyces RNA Messenger Schizosaccharomyces pombe Proteins Nuclear protein Genes Suppressor Gene |
| Zdroj: | Journal of microbiology (Seoul, Korea). 46(4) |
| ISSN: | 1225-8873 |
| Popis: | We have isolated Glel homologue (named as spglel) as a partial multicopy suppressor of the synthetic lethality of rael-167 elfl-21 in fission yeast Schizosaccharomyces pombe. The spglel is also able to complement partially temperature-sensitive phenotype of rael-167 only at a lower restrictive temperature. The spglel gene contains one intron and encodes a 480 amino-acid protein with predicted molecular weight of 56.2 kDa. We showed that spglel gene is essential for vegetative growth and functional Glel-GFP protein is localized mainly in NPC. The accumulation of poly(A)(+) RNA in the nucleus is exhibited when expression of spglel is repressed or over-expressed. These results suggest that the spGle1 protein is also involved in mRNA export in fission yeast. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink