Identification of trypanosomes in wild animals from Southern Cameroon using the polymerase chain reaction (PCR)
| Autor: | Flobert Njiokou, S.W. Nkinin, Stéphane Herder, Gustave Simo |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Trypanosoma
reservoir Veterinary medicine Trypanosoma congolense Trypanosoma brucei gambiense sleeping sickness Veterinary (miscellaneous) Trypanosoma brucei brucei forest belt Animals Wild Buffy coat Polymerase Chain Reaction lcsh:Infectious and parasitic diseases law.invention Species Specificity Trypanosomiasis law Animals Parasite hosting lcsh:RC109-216 Cameroon T. b. gambiense Polymerase chain reaction Cercopithecus nictitans Disease Reservoirs Genetta servalina Cercocebus torquatus Atherurus africanus biology DNA Protozoan Cephalophus dorsalis biology.organism_classification Insect Vectors wild animal PCR Infectious Diseases Insect Science Animal Science and Zoology Parasitology |
| Zdroj: | Parasite, Vol 9, Iss 4, Pp 345-349 (2002) |
| ISSN: | 1776-1042 1252-607X |
| DOI: | 10.1051/parasite/2002094345 |
| Popis: | One possible explanation of the maintenance of many historical foci of sleeping sickness in Central Africa could be the existence of a wild animal reservoir. In this study, PCR was used to detect the different trypanosome species present in wild animal captured by hunters in the southern forest belt of Cameroon (Bipindi). Trypanosomes were also detected by a parasitological method (Quantitative buffy coat : QBC). Parasite could not be isolated in culture medium (Kit for in vitro isolation : KIVI). Specific primers of T. brucei s.l., T. congolense forest type, T. congolense savannah type, T. vivax, T. simiae and T. b. gambiense group 1 were used to identify parasites in the blood of 164 animals belonging to 24 different species including ungulates, rodents, pangolins, carnivores, reptiles and primates. Of the 24 studied species, eight were carrying T. b. gambiense group 1. Those parasites pathogenic to man were found in monkeys (Cercocebus torquatus and Cercopithecus nictitans), in ungulates (Cephalophus dorsalis and C. monticola), in carnivores (Nandinia binotata and Genetta servalina) and in rodents (Cricetomys gambianus and Atherurus africanus). 13 species (54 %) were carrying T. brucei s.l. identified as non-gambiense group 1. |
| Databáze: | OpenAIRE |
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