Abnormalities of CD4+ T cell subpopulations in ANCA-associated vasculitis
| Autor: | Wilhelm H. Schmitt, Rainer Birck, F. J. Van Der Woude, Ruediger Waldherr, Smaragdi Marinaki, Annette Breedijk, Benito A. Yard, Anna-Isabelle Kälsch, Irmgard Neumann, Peter P. Grimminger |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Vasculitis medicine.medical_specialty medicine.medical_treatment T cell Immunology Biology Kidney Lymphocyte Activation Antibodies Antineutrophil Cytoplasmic Immunophenotyping Interferon-gamma Interleukin 21 Internal medicine Clinical Studies medicine Humans Immunology and Allergy RNA Messenger IL-2 receptor Cells Cultured Aged Aged 80 and over Interleukins FOXP3 CD28 Forkhead Transcription Factors Receptors Interleukin-2 T lymphocyte Middle Aged Kidney Transplantation DNA-Binding Proteins Endocrinology Cytokine medicine.anatomical_structure Leukocyte Common Antigens Female Cytokine secretion |
| Zdroj: | Clinical and Experimental Immunology. 140:181-191 |
| ISSN: | 1365-2249 0009-9104 |
| Popis: | SummaryIn patients with ANCA-associated vasculitis (AAV), CD25 expression is increased on circulating T cells. Although in animal experiments the role of CD4+ CD25+ T-regulatory-cells (Treg) in protection against autoimmunity is well established, the role of these cells in AAV is unknown. To investigate the hypothesis that an increased expression of CD25 on T cells is related to persistent T cell activation and not to disturbances in Treg cells in AAV (34 patients, six of them after renal transplantation), we investigated CD25 expression in different subpopulations of CD4+ cells and FOXP3 mRNA expression by reverse transcription-polymerase chain reaction (RT-PCR). In addition, T cell proliferation and cytokine secretion after stimulation with anti-CD3 and anti-CD28 and intracellular cytokine production after stimulation with phorbol myristate acetate (PMA)-ionomycin was determined. Controls were non-vasculitic renal transplant patients (n = 9) and healthy controls (HC) (n = 13). In AAV the total number of lymphocytes, CD4+ lymphocytes and the percentage of naive T cells are lower than in HC and RTX. An increased percentage of CD25+ cells was found in AAV and AAV/RTX, irrespective of disease activity, but not in HC or RTX. This was confined to the naive (CD4+ CD45RBhigh) population only. FOXP3 mRNA expression in CD4+ T cells did not differ between AAV patients and healthy controls. In vitro T cell proliferation was enhanced in AAV patients compared to HC (P |
| Databáze: | OpenAIRE |
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