Cell-cell fusion as a mechanism of DNA exchange in cancer
| Autor: | Jack D. Bui, Stephen C. Searles, Endi K. Santosa |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Chemistry cell-cell fusion Cell chemoresistance Cancer Extracellular vesicle cancer heterogeneity medicine.disease In vitro Cell biology 03 medical and health sciences Haematopoiesis 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cancer cell Extracellular medicine clonal diversity aneuploidy Intracellular Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.23715 |
| Popis: | Cell-cell fusion describes the process by which two cells combine their plasma membranes and become a single cell, possessing and retaining certain genetic information from each parent cell. Here, using a Cre-loxP-based method initially developed to investigate extracellular vesicle targeting, we found that cancer cells spontaneously and rapidly deliver DNA to non-cancer cells in vitro via a cell-cell fusion event. The resulting hybrid cells were aneuploid and possessed enhanced clonal diversity and chemoresistance compared to non-hybrid cancer cells. We also observed cell-cell fusion to occur in vivo between melanoma cells and non-cancer cells of both hematopoietic and non-hematopoietic lineages. These findings suggest that cell-cell fusion occurs during the natural progression of cancer and show that this mechanism has the potential to cause massive genomic alterations that are observed in cancer. Furthermore, these findings somewhat contradict recent publications suggesting that the Cre-loxP method measures only extracellular vesicle-mediated intercellular communication. |
| Databáze: | OpenAIRE |
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