Performance of the commercially available SERION ELISA classic Echinococcus IgG test for the detection of cystic echinococcosis in clinical practice
| Autor: | Sarink, M. J., Koelewijn, R., Slingerland, B. C.G.C., Tielens, A. G.M., van Genderen, P. J.J., van Hellemond, J. J., LS Veterinaire biochemie, Dep Biochemie en Celbiologie |
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| Přispěvatelé: | LS Veterinaire biochemie, Dep Biochemie en Celbiologie, Medical Microbiology & Infectious Diseases |
| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
diagnosis 030231 tropical medicine Antibodies Helminth serology Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Gastroenterology 030308 mycology & parasitology Serology 03 medical and health sciences 0302 clinical medicine Echinococcosis Internal medicine medicine Animals Humans Echinococcus granulosus 0303 health sciences biology Echinococcus IgG Cystic echinococcosis General Medicine biology.organism_classification Serum samples medicine.disease cystic echinococcosis Clinical Practice Parasitology Antigens Helminth Immunoglobulin G ELISA Animal Science and Zoology Reagent Kits Diagnostic |
| Zdroj: | Journal of Helminthology, 93(5), 636-639. Cambridge University Press Journal of Helminthology, 93(5), 636. Cambridge University Press |
| ISSN: | 1475-2697 0022-149X |
| Popis: | Diagnosis of cystic echinococcosis (CE) is at present mainly based on imaging techniques. Serology has a complementary role, partly due to the small number of standardized and commercially available assays. Therefore we examined the clinical performance of the SERION ELISA classic Echinococcus IgG test. Using 10 U/ml as a cut-off point, and serum samples from 50 CE patients and 105 healthy controls, the sensitivity and specificity were 98.0% and 96.2%, respectively. If patients with other infectious diseases were used as negative controls, the specificity decreased to 76.9%, which causes poor positive predictive values. However, if results between 10 and 15 U/ml are classified as indecisive, the specificity of positive results (≥15 U/ml) increased to 92.5% without greatly affecting the sensitivity (92.0%). Using this approach in combination with imaging studies, the SERION ELISA classic Echinococcosis IgG test can be a useful aid in the diagnosis of CE. |
| Databáze: | OpenAIRE |
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