Early activation of fibrogenesis in transplanted kidneys: A study on serial renal biopsies
| Autor: | Franca Anglani, Luciana Bonfante, Giuseppe Scaparrotta, Lucrezia Furian, Dorella Del Prete, Marialuisa Valente, Paolo Rigotti, Daniela Vianello, Angela D'Angelo, R. Graziotto, Giovanni Gambaro, Emilia Tiralongo, Monica Ceol |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Biopsy Messenger Clinical Biochemistry Gene Expression Biology Transplant Kidney Pathology and Forensic Medicine Masson's trichrome stain Renin-Angiotensin System Transforming Growth Factor beta1 Fibrosis Trichrome Gene expression Immunohistochemistry RAS Renal biopsies TGFβ1 Humans Kidney Transplantation Middle Aged RNA Messenger Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Molecular Biology 2734 medicine Settore MED/14 - NEFROLOGIA Kidney transplantation medicine.diagnostic_test Anatomical pathology medicine.disease fibrogenesis Transplantation medicine.anatomical_structure RNA transplantation kidney |
| Popis: | Background/aims In kidney transplants, the renin–angiotensin system (RAS) is involved in systemic and local changes that may induce fibrosis. Our aim was to use gene expression and immunohistochemical analysis to investigate the RAS and several factors involved in the fibrogenic cascade in allograft biopsies. Methods We considered 43 donor biopsies (T0), 18 biopsies obtained for diagnostic purposes (Td) and 24 protocol biopsies (Tp) taken 2 months after transplantation in patients with stable renal function. Morphometric αSMA and TGFβ1 analysis, and Masson's Trichrome staining were performed. mRNA levels of angiotensinogen, renin, ACE, AT1-R, AT2-R, TGFβ1, BMP-7, Coll III, fibronectin and αSMA were analyzed by real-time RT/PCR. MDRD a year after the transplant was also considered. Results Significantly higher levels of AT1-R and αSMA transcripts were found in Tp than in T0. Regression analysis showed significant TGFβ1-independent positive correlations between RAS and matrix components in T0 and Tp, but more evident in Tp, where a positive correlation between TGFβ1 and Masson's Trichrome stained areas was also seen. Conclusion Our results suggest that RAS and TGFβ1-related fibrogenic loops are activated as early as 2 months after kidney transplantation. |
| Databáze: | OpenAIRE |
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