Lack of Apoptosis in Listeria monocytogenes -Infected Thymocytes from Mice Fed with Dietary Lipids
| Autor: | Gerardo Alvarez de Cienfuegos, Alfonso Ruiz-Bravo, Manuel A. de Pablo, María A. Puertollano, Maria Jimenez-Valera, Elena Puertollano |
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| Rok vydání: | 2004 |
| Předmět: |
Proteasome Endopeptidase Complex
T-Lymphocytes Dietary lipid Cell Culture Techniques Apoptosis DNA Fragmentation Thymus Gland Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Mice Random Allocation Fish Oils Immune system Dietary Fats Unsaturated Listeria monocytogenes Multienzyme Complexes medicine Animals Plant Oils Cytotoxic T cell Olive Oil Inhibitory effect chemistry.chemical_classification Mice Inbred BALB C L-Lactate Dehydrogenase Caspase 3 General Medicine Cysteine Endopeptidases chemistry Caspases Coconut Oil DNA fragmentation Polyunsaturated fatty acid |
| Zdroj: | Current Microbiology. 48:373-378 |
| ISSN: | 1432-0991 0343-8651 |
| Popis: | The potential action of certain fatty acids has been studied since the early 1970s. Numerous effects on immune system functions have been related to dietary lipid administration; therefore, several of them have been applied in the treatment of inflammatory disorders. Nevertheless, n-3 polyunsaturated fatty acids may affect host resistance to infectious diseases. In addition, several studies have demonstrated that certain fatty acids are involved in apoptosis induction. Here, we have examined the action of different dietary lipids on the promotion of apoptosis in thymocytes from mice fed with dietary lipids and infected with Listeria monocytogenes. Thus, L. monocytogenes promoted an important cytotoxic effect in all of the groups, but it did not increase the percentage of DNA fragmentation. Similarly, an important increase of caspase-3 activity was demonstrated in OO and FO groups, but infection with L. monocytogenes exerted an inhibitory effect. Finally, L. monocytogenes did not modify proteasome activity among groups fed with dietary lipids. On the basis of this preliminary study, we can state that the infection of thymocytes from mice fed with dietary lipids does not promote a synergistic effect in the induction of apoptosis. Hence, these results may partially serve to elucidate the immune mechanisms involved in cells from mice fed with dietary lipids in an infectious process. |
| Databáze: | OpenAIRE |
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