In-capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection
| Autor: | Leena Suntornsuk, Nantana Nuchtavorn, Pornpan Prapatpong, Mirek Macka |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Chromatography
Molecular Structure Rimantadine Capillary action Adamantane 010401 analytical chemistry Electrophoresis Capillary Filtration and Separation 010402 general chemistry Fluorescamine 01 natural sciences 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Capillary electrophoresis chemistry Reagent medicine Spectrophotometry Ultraviolet Acetonitrile Derivatization medicine.drug |
| Zdroj: | Journal of Separation Science. 41:3764-3771 |
| ISSN: | 1615-9306 |
| Popis: | In-capillary derivatization using fluorescamine as the labeling reagent was proposed to enhance the detectability of adamantine drugs (memantine, amantadine and rimantadine) by spectrophotometric detection. Fluorescamine and the drugs were delivered to the capillary electrophoresis instrument at a ratio of 10:1 by zone injection. The derivatization reaction occurred following the application of voltage (20 kV). The derivatized products, hydrolyzed- fluorescamine and excess fluorescamine were separated in 7 min using 100 mM borate buffer (pH 10.0) containing 0.1% w/v of Brij®-35 and 20% v/v of acetonitrile. Validation data showed good linearity (r2 > 0.98), precision (%RSDs |
| Databáze: | OpenAIRE |
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