FTIR microspectroscopy of malignant fibroblasts transformed by mouse sarcoma virus
| Autor: | Jagannathan Ramesh, Vitaly Erukhimovitch, Shaul Mordechai, Mahmoud Huleihel, Marina Talyshinsky, Ahmad Salman |
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| Rok vydání: | 2003 |
| Předmět: |
Biophysics
Bone Marrow Cells Biology Biochemistry Phosphates Absorbance Sarcoma Viruses Murine chemistry.chemical_compound Mice Reference Values Spectroscopy Fourier Transform Infrared medicine Biomarkers Tumor Tumor Cells Cultured Animals Cluster Analysis RNA Neoplasm Primary cell Fibroblast RNA DNA Neoplasm Fibroblasts Cell Transformation Viral Virology Molecular biology Amides Mouse sarcoma virus medicine.anatomical_structure chemistry Cell culture Microspectrophotometry Bone marrow Rabbits DNA |
| Zdroj: | Journal of biochemical and biophysical methods. 55(2) |
| ISSN: | 0165-022X |
| Popis: | Fourier transform infrared microspectroscopy (FTIR-MSP), which is based on the characteristic molecular vibrational spectra of cells, was used to investigate spectral differences between normal primary rabbit bone marrow (BM) cells and bone marrow cells transformed (BMT) by murine sarcoma virus (MuSV). Primary cells, rather than cell lines, were used for this research because primary cells are similar to normal tissue cells in most of their characteristics. Our results showed dramatic changes in absorbance between the control cells and MuSV124-transformed cells. Various biological markers, such as the phosphate level and the RNA/DNA obtained, based on the analysis of the FTIR-MSP spectra, also displayed significant differences between the control and transformed cells. Preliminary results suggested that the cluster analysis performed on the FTIR-MSP spectra yielded 100% accuracy in classifying both types of cells. |
| Databáze: | OpenAIRE |
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