Determination of the structure of [Nle7]-endothelin by 1H NMR
| Autor: | Dung Le-Nguyen, Bruno Perly, Eve Mahe, Laurent Chiche, Patrick Berthault, Philippe Sizun, André Aumelas |
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| Rok vydání: | 2009 |
| Předmět: |
Deuterium NMR
Acetonitriles Magnetic Resonance Spectroscopy Protein Conformation Chemistry Carbon-13 NMR satellite Endothelins Molecular Sequence Data Analytical chemistry Water Nuclear magnetic resonance crystallography Nuclear magnetic resonance spectroscopy Fluorine-19 NMR Biochemistry Solutions Proton NMR Physical chemistry Dimethyl Sulfoxide Transverse relaxation-optimized spectroscopy Amino Acid Sequence Two-dimensional nuclear magnetic resonance spectroscopy |
| Zdroj: | International Journal of Peptide and Protein Research. 37:315-324 |
| ISSN: | 0367-8377 |
| Popis: | [Nle7]-endothelin was synthesized and studied by 1H NMR and distance geometry calculations. The NMR study was performed first in DMSO-d6 and then in 50% acetonitrile/water since this peptide aggregates in pure water. In both cases, all spin systems were identified and assigned with the aid of two-dimensional spectroscopy (2D): COSY (for scalar couplings) and NOESY (for dipolar couplings). On the basis of the acetonitrile/water NMR parameters, and using the DISGEO program, a three-dimensional structure of [Nle7]-endothelin is proposed and discussed. |
| Databáze: | OpenAIRE |
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