Expression and role of Bcl-2 in rat blastocysts exposed to high D-glucose
| Autor: | René De Hertogh, Isabelle Donnay, Patrick Van Der Smissen, Ivo Vanderheyden, Pierre J. Courtoy, Sabine Cordi, S. Pampfer, Anne Van Cauwenberge, Henri Alexandre |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Karyolysis
Endocrinology Diabetes and Metabolism Immunocytochemistry In situ hybridization Biology Culture Techniques Gene expression Internal Medicine medicine Animals Blastocyst RNA Messenger Fragmentation (cell biology) Rats Wistar Dose-Response Relationship Drug Oligonucleotides Antisense Molecular biology In vitro Rats medicine.anatomical_structure Glucose Proto-Oncogene Proteins c-bcl-2 Apoptosis Immunology Female |
| Zdroj: | Diabetes. 50(1) |
| ISSN: | 0012-1797 |
| Popis: | Bcl-2 mRNA expression was detected in rat blastocysts by in situ hybridization. The distribution of mRNA expression was rather heterogenous, with ∼2% of high-expressing cells. In vitro exposure to 28 mmol/l D-glucose for 24 h resulted in a significant increase in the proportion of these cells compared with control embryos in either 6 mmol/l D-glucose or 28 mmol/l D+L-glucose. Heterogeneity in the expression of Bcl-2 was also observed at the protein level by immunocytochemistry. Exposure to 28 mmol/l D-glucose significantly increased the incidence of chromatin degradation (karyolysis) and nuclear fragmentation (karyorhexis), two nuclear markers of apoptosis in rat blastocysts. When two different antisense oligodeoxynucleotides designed to block Bcl-2 expression were added to 28 mmol/l D-glucose, the incidence of karyolysis (but not karyorhexis) was increased compared with embryos in 28 mmol/l D-glucose alone. These data suggest that Bcl-2 is involved in the protective response against the induction of karyolysis in blastocysts on their exposure to high concentrations of D-glucose in vitro, whereas karyorhexis appears to result from the activation of an intracellular pathway that is independent of Bcl-2. |
| Databáze: | OpenAIRE |
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