Anti-placental growth factor antibody ameliorates hyperoxia-mediated impairment of lung development in neonatal rats

Autor: Zhang, Zhiqun, Zhong, Ying, Li, Xiaoxia, Huang, Xianmei, Du, Lizhong
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Placental growth factor
Medicine (General)
Physiology
Biochemistry
0302 clinical medicine
Pregnancy
General Pharmacology
Toxicology and Pharmaceutics
Biology (General)
Receptor
Hyperoxia
medicine.diagnostic_test
General Neuroscience
Antibodies
Monoclonal
Lung Injury
General Medicine
respiratory system
medicine.anatomical_structure
030220 oncology & carcinogenesis
cardiovascular system
Newborn rats
Immunohistochemistry
Female
lipids (amino acids
peptides
and proteins)
medicine.symptom
Research Article
medicine.medical_specialty
endocrine system
QH301-705.5
Immunology
Biophysics
Ocean Engineering
Lung injury
03 medical and health sciences
R5-920
Internal medicine
medicine
Animals
Autoantibodies
Placenta Growth Factor
Lung
business.industry
Cell Biology
medicine.disease
Bronchopulmonary dysplasia
Rats
respiratory tract diseases
Disease Models
Animal
030104 developmental biology
Endocrinology
Bronchoalveolar lavage
Anti-PGF antibody
Animals
Newborn
Microscopy
Electron
Scanning
business
Lung tissue injury
Zdroj: Brazilian Journal of Medical and Biological Research, Volume: 53, Issue: 2, Article number: e8917, Published: 24 JAN 2020
Brazilian Journal of Medical and Biological Research v.53 n.2 2020
Brazilian Journal of Medical and Biological Research
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Brazilian Journal of Medical and Biological Research, Vol 53, Iss 2 (2020)
Popis: This study investigates the effect of the overexpression of the placental growth factor (PGF) and hyperoxia on lung development and determines whether anti-PGF antibody ameliorates hyperoxia-mediated impairment of lung development in newborn rats. After exposure to normoxic conditions for seven days, newborn rats subjected to normoxia were intraperitoneally or intratracheally injected with physiological saline, adenovirus-negative control (Ad-NC), or adenovirus-PGF (Ad-PGF) to create the Normoxia, Normoxia+Ad-NC, and Normoxia+Ad-PGF groups, respectively. Newborn rats subjected to hyperoxia were intraperitoneally injected with physiological saline or anti-PGF antibodies to create the Hyperoxia and Hyperoxia+anti-PGF groups, respectively. Our results revealed significant augmentation in the levels of PGF and its receptor Flt-1 in the lung tissues of newborn rats belonging to the Normoxia+Ad-PGF or Hyperoxia groups. PGF overexpression in these groups caused lung injury in newborn rats, while anti-PGF antibody treatment significantly cured the hyperoxia-induced lung injury. Moreover, PGF overexpression significantly increased TNF-α and Il-6 levels in the bronchoalveolar lavage (BAL) fluid of the Normoxia+Ad-PGF and Hyperoxia groups. However, their levels were significantly reduced in the BAL fluid of the Hyperoxia+anti-PGF group. Immunohistochemical analysis revealed that PGF overexpression and hyperoxia treatment significantly increased the expression of the angiogenesis marker, CD34. However, its expression was significantly decreased upon administration of anti-PGF antibodies (compared to the control group under hyperoxia). In conclusion, PGF overexpression impairs lung development in newborn rats while its inhibition using an anti-PGF antibody ameliorates the same. These results provided new insights for the clinical management of bronchopulmonary dysplasia in premature infants.
Databáze: OpenAIRE
Externí odkaz: